Objective To construct a eukaryotic expression vector of Trappin-2 gene and investigate its effect on the proliferation of HaCaT cells and transwell psoriatic skin organ culture models.   Methods The pIRES2-EGFP-Trappin-2 expression vector was constructed by DNA recombinant technique and transfected into HeLa cells with lipofectamine

TM

2000

then the supernatant containing Trappin-2 was added to HaCaT cells and transwell psoriatic skin organ culture models. 

3

H-TdR incorporation

MTT method

cell cycle analysis and expression of Ki67 were used to examine their proliferation.   Results Trappin-2 sign

ificantly inhibited the proliferation of HaCaT cells. Trappin-2 significantly decreased the proportion of the cells in G

2

+S phase. Trappin-2 reduced the Ki67 expression in psoriatic skin models.   Conclusion pIRES2-EGFP-Trappin-2 could be successfully transfected into HeLa cells

and the expressed Trappin-2 inhibits the proliferation of HaCaT cells and psoriatic skin models.