Effect of TNF-α on blood-labyrinth barrier permeability of microvascular endothelial cells from cochlear stria vascularis of guinea pig

李琪; 张学渊

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Effect of TNF-α on blood-labyrinth barrier permeability of microvascular endothelial cells from cochlear stria vascularis of guinea pig

更新时间：2026-01-09

- Effect of TNF-α on blood-labyrinth barrier permeability of microvascular endothelial cells from cochlear stria vascularis of guinea pig
- Journal of Army Medical University Vol. 30, Issue 2, Pages: 174-175(2008)
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  CLC： R322.13;R322.923;R329.27
- Published：2008
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李琪,张学渊.TNF-α对体外培养豚鼠耳蜗血管纹微血管内皮细胞通透性的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):174-175.

　LI Qi,ZHANG Xue-yuan.Effect of TNF-α on blood-labyrinth barrier permeability of microvascular endothelial cells from cochlear stria vascularis of guinea pig[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):174-175.
李琪,张学渊.TNF-α对体外培养豚鼠耳蜗血管纹微血管内皮细胞通透性的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):174-175. DOI：

　LI Qi,ZHANG Xue-yuan.Effect of TNF-α on blood-labyrinth barrier permeability of microvascular endothelial cells from cochlear stria vascularis of guinea pig[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):174-175. DOI：

摘要

目的研究TNF-α对体外培养豚鼠耳蜗血管纹微血管内皮细胞通透性的影响。方法体外分离培养豚鼠耳蜗血管纹微血管内皮细胞并建立耳蜗血管纹微血管内皮细胞通透性体外模型，实验分TNF-α组和对照组。依据作用时间和浓度不同，检测其对伊文思蓝的通透率变化。结果 TNF-α能显著增加耳蜗血管纹微血管内皮细胞的通透性，使伊文思蓝的通透率显著升高(P0.01），且随TNF-α浓度升高和作用时间的延长而升高。结论 TNF-α可以显著的增加耳蜗血管纹微血管内皮细胞的通透性。

Abstract

Objective To investigate the mechanism of TNF-α on regulating the blood-labyrinth barrier permeability of cochlea of guinea pig. Methods The stria vascularis was acquired from guinea pigs and the endothelial cells were cultured of with purified to establish the models of the blood-labyrinth barrier according to our previous researches. The models were treated with TNF-α (0.05

0.1

0.2 ng/ml) for 30

90 min or with serum-free DMEM as control. Then their permeability was measured by Evens blue technique. Results TNF-α increases Evans blue transfer across endothelial cell monolayer (P0.01). The filtration rate of Evans blue across the endothelial cell monolayer increased positively with prolonged treatment time or the increasing TNF-α concentration. Conclusion TNF-α enhances the permeability of blood-labyrinth barrier in guinea pig.

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