Establishment and biological characteristics of human colon carcinoma multidrug-resistant cell line HT29/L-OHP

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Establishment and biological characteristics of human colon carcinoma multidrug-resistant cell line HT29/L-OHP

更新时间：2026-01-09

- Establishment and biological characteristics of human colon carcinoma multidrug-resistant cell line HT29/L-OHP
- Journal of Army Medical University Vol. 30, Issue 9, Pages: 840-843(2008)
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  CLC： R329.2;R73-354;R735.35
- Published：2008
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向征,张才全,汤为学.人结肠癌耐药细胞系HT29/L-OHP的建立及其生物学特性[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):840-843.

　XIANG Zheng,ZHANG Cai-quan,TANG Wei-xue.Establishment and biological characteristics of human colon carcinoma multidrug-resistant cell line HT29/L-OHP[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(09):840-843.
向征,张才全,汤为学.人结肠癌耐药细胞系HT29/L-OHP的建立及其生物学特性[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):840-843. DOI：

　XIANG Zheng,ZHANG Cai-quan,TANG Wei-xue.Establishment and biological characteristics of human colon carcinoma multidrug-resistant cell line HT29/L-OHP[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(09):840-843. DOI：

摘要

目的建立人结肠癌奥沙利铂耐药细胞系HT29/L-OHP

探讨结肠癌对奥沙利铂（Oxaliplatin，L-OHP）产生耐药的特征规律和耐药的机制。方法采用逐步递增L-OHP浓度，间歇作用体外诱导法培养建立了人结肠癌L-OHP 耐药细胞模型HT29/L-OHP。MTT法测定生长曲线、交叉耐药性和耐药指数；并用光镜、电镜、流式细胞仪（FCM）等方法观察其一般生物学特性的改变。结果历时3个月建成了L-OHP耐药细胞模型HT29/L-OHP，其耐药性能稳定，耐药指数为10.64；并且与5-氟尿嘧啶、长春新碱、阿霉素等多种抗癌药有不同程度的交叉耐药性。HT29/L-OHP的细胞形态及染色体数目发生了改变；体外细胞群体倍增时间较亲代细胞延长29.71 h；细胞周期分析结果显示其S期与G

期细胞减少、G

/M期细胞增多。结论 HT29/L-OHP细胞是模拟临床用药特点建立的耐药性较稳定的多药耐药细胞，具有耐药细胞的基本生物学特性，是研究L-OHP耐药机制及筛选逆转剂的理想细胞模型。

Abstract

Objective To establish a drug-resistant human colon cancer cell line to oxaliplatin(L-OHP) based on cell line HT29. Methods A resistant cell line-HT29/L-OHP was established by gradually increasing the dose of L-OHP and intermittent administration. The growth curves

multidrug resistance and resistance index of HT29/L-OHP cell line to anticancer agents were detected by MTT assay. The changes of its biological characteristics were determined by light microscopy

electron microscopy

and flow cytometry. Results HT29/L-OHP cell line was established aft

er 3 months

which had stable resistance to L-OHP and a resistance index of 10.64. HT29/L-OHP cells exhibited cross resistance to many other chemotherapeutic agents. As compared with parental cells

the morphological and chromatosome number of HT29/L-OHP were changed; its doubling time was prolonged; and the number of cells in S phase and G

phase were decreased while in G

/M phase increased. Conclusion HT29/L-OHP cell line shows the typical multidrug-resistant phenotype.

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