Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma

孙梯业; 赵永亮; 颜伟; 石彦; 曾冬竹; 余佩武

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Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma

更新时间：2026-01-09

- Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma
- Journal of Army Medical University Vol. 30, Issue 2, Pages: 161-165(2008)
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  CLC： R73-362;R73-37;R735.2
- Published：2008
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孙梯业,赵永亮,颜伟,石彦,曾冬竹,余佩武.CXCR4在VEGF-C介导的胃癌淋巴道转移中的作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):161-165.

　SUN Ti-ye,ZHAO Yong-liang,YAN Wei,SHI Yan,ZENG Dong-zhu,YU Pei-wu.Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):161-165.
孙梯业,赵永亮,颜伟,石彦,曾冬竹,余佩武.CXCR4在VEGF-C介导的胃癌淋巴道转移中的作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):161-165. DOI：

　SUN Ti-ye,ZHAO Yong-liang,YAN Wei,SHI Yan,ZENG Dong-zhu,YU Pei-wu.Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):161-165. DOI：

摘要

目的探索趋化因子受体CXCR4和VEGF-C与胃癌淋巴道转移的关系，为研究干预胃癌淋巴转移的新方法提供理论依据。方法采用RT-PCR方法检测86例胃癌组织中趋化因子受体CXCR4 mRNA的表达及VEGF-C mRNA的表达，探索其与胃癌淋巴结转移的关系；Boyden趋化小室法检测CXCR4不同表达状态胃癌细胞的趋化活性和趋化抑制性。结果 86例胃癌组织均有不同程度的CXCR4 mRNA和VEGF-C mRNA的表达。53例CXCR4 mRNA呈高表达，表达率为61.63％，VEGF-C mRNA高表达率为56.98％(49/86)；VEGF-C mRNA高表达组中的CXCR4 mRNA的表达率也较高；CXCR4 mRNA的表达与胃癌淋巴结转移呈正相关(P0.05)；CXCR4的配体SDF-l对胃癌细胞的平均趋化率为81％，经抗CXCR4单克隆抗体处理后癌细胞的平均趋化率下降至30％，CXCR4被特异性抗体封闭前后的癌细胞趋化活性有显著性差异(P0.05)。结论胃癌CXCR4的表达和VEGF-C的表达与胃癌淋巴结转移呈正相关；CXCR4的功能状态影响胃癌细胞的迁移活性。通过干预趋化因子受体CXCR4和VEGF-C的活性可能成为阻断胃癌淋巴转移的新靶点。

Abstract

Objective To explore the role of chemokine receptor CXCR4 in lymphatic metastasis induced by VEGF-C in gastric carcinoma. Methods The mRNA expressions of CXCR4 and VEGF-C were determined by reverse transcription PCR (RT-PCR) in 86 cases of primary breast carcinoma

and the migration and inhibition response of CXCR4 to chemokine stromal derived factor-1 (SDF-1 or CXCL12) were detected by Boyden chemotaxis assay. Results Out of 86 cases

53 cases presented high expression levels of CXCR4 (61.63%

53/86)

49 cases showed high expression levels of VEGF-C mRNA (56.98％

49/86). The cases with higher expression of VEGF-C mRNA presented higher expression of CXCR4 mRNA. The expression of CXCR4 mRNA was positively correlated with lymph node metastasis in primary gastric carcinoma (P0.05). The average chemotaxis activity of CXCR4 to SDF-1 was evaluated as 81% in gastric cancer cells

but dropped to 30% after using anti-CXCR4 monoclonal antibody

with significant difference (P0.05). Conclusion There is a positive correlation between lymphatic metastasis and the expression of CXCR4 and VEGF-C mRNA in primary gastric carcinoma. The functional status of CXCR4 affects the chemotaxis of gastric cancer cells.

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