Amlodipine inhibits in vitro invasion of human breast carcinoma cells MDA-MB-231

徐兴华; 孙文娟; 周岐新; 马凤云

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Amlodipine inhibits in vitro invasion of human breast carcinoma cells MDA-MB-231

更新时间：2026-01-09

- Amlodipine inhibits in vitro invasion of human breast carcinoma cells MDA-MB-231
- Journal of Army Medical University Vol. 30, Issue 4, Pages: 340-342(2008)
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  CLC： R73-361;R730.23;R737.9
- Published：2008
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徐兴华,孙文娟,周岐新,马凤云.氨氯地平对人乳腺癌细胞株MDA-MB-231侵袭影响的体外实验[J].陆军军医大学学报(原第三军医大学学报),2008,30(04):340-342.

　XU Xing-hua,SUN Wen-juan,ZHOU Qi-xin,MA Feng-yun.Amlodipine inhibits in vitro invasion of human breast carcinoma cells MDA-MB-231[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(04):340-342.
徐兴华,孙文娟,周岐新,马凤云.氨氯地平对人乳腺癌细胞株MDA-MB-231侵袭影响的体外实验[J].陆军军医大学学报(原第三军医大学学报),2008,30(04):340-342. DOI：

　XU Xing-hua,SUN Wen-juan,ZHOU Qi-xin,MA Feng-yun.Amlodipine inhibits in vitro invasion of human breast carcinoma cells MDA-MB-231[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(04):340-342. DOI：

摘要

目的探讨氨氯地平对人乳腺癌高转移细胞株MDA-MB-231体外侵袭的影响及其相关机制。方法用8.34 μmol/L氨氯地平处理肿瘤细胞，以人工重组基底膜侵袭小室（Transwell）观察氨氯地平对MDA-MB-231细胞侵袭的影响；免疫细胞化学方法检测血管内皮生长因子（vascular endothelial growth factor

VEGF）和基质金属蛋白酶-9〖JP2〗（matrix metallo-proteinase，MMP-9）的表达。结果 8.34 μmol/L的氨氯地平可显著抑制MDA-MB-231细胞的侵袭能力，侵袭抑制率为36.27%；免疫细胞化学检测结果显示，MMP-9和VEGF蛋白的表达在MDA-MB-231细胞中亦明显降低结论氨氯地平对MDA-MB-231细胞体外侵袭具有明显的抑制作用，此作用与降低肿瘤细胞的MMP-9和VEGF的表达有关。

Abstract

Objective To investigate the mechanism that Amlodipine inhibits the invasion of human breast cancer cell line MDA-MB-231. Methods MDA-MB-231 cells was treated by 8.34 μmol/L Amlodipine for 72 h. The invasion activity of MDA-MB-231 cells was evaluated by using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane (Matrigel). The expressions of MMP-9 and VEGF in MDA-MB-231 cells were examined by immunohistochemistry. Results Amlodipine significantly inhibited the invasion activity of MDA-MB-231 cells

and the inhibitory rate was (36.27±6.39)%. The expressions of MMP-9 and VEGF in MDA-MB-231 cells decreased obviously by Amlodipine. Conclusion Amlodipine exhibits the invasion activity of breast cancer cell line MDA-MB-231 and it is related to the reduction of MMP-9 and VEGF expressions.

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