TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions

陈婧; 李毅; 谭畅; 刘湘; 黄爱龙; 汤华

您当前的位置：

首页 >

文章列表页 >

TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions

更新时间：2026-01-09

- TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions
- Journal of Army Medical University Vol. 30, Issue 9, Pages: 834-836(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R373.21;R392.11;R392.2
- Published：2008
- 稿件说明：
移动端阅览
陈婧,李毅,谭畅,刘湘,黄爱龙,汤华.TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):834-836.

　CHEN Jing,LI Yi,TAN Chang,LIU Xiang,HUANG Ai-long,TANG Hua.TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(09):834-836.
陈婧,李毅,谭畅,刘湘,黄爱龙,汤华.TIA-1通过影响肝细胞转录因子的活性调节HBV的e抗原和s抗原表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(09):834-836. DOI：

　CHEN Jing,LI Yi,TAN Chang,LIU Xiang,HUANG Ai-long,TANG Hua.TIA-1 may interact with liver-enriched transcription factors and regulate HBV s and e antigen expressions[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(09):834-836. DOI：

摘要

目的证明TIA-1分子具有调节HBV基因表达的功能。方法 TIA-1真核表达质粒pcDNA3-TIA-1，用脂质体转染HepG 2.2.15细胞后，ELISA检测e和s抗原的表达；RT-PCR检测与HBV复制有关的转录因子HNF1、 HNF2、 HNF3、HNF4、 RXRα、 PPARα、C/EBP的表达。结果与正常的HepG 2.2.15细胞相比，转染pcDNA3-TIA-1质粒的HepG 2.2.15细胞，其e抗原的表达量明显的增高，而s抗原表达量明显的降低。与正常的HepG 2.2.15细胞相比，转染pcDNA3-TIA-1质粒的HepG 2.2.15细胞中的HNF1、 HNF2、 RXRα mRNA含量有明显的下降。结论 TIA-1蛋白能通过影响与HBV复制有关的转录因子活性，调节HBV e抗原和s抗原的表达。

Abstract

Objective To identify TIA-1 function in regulating HBV replication. Methods pcDNA3-TIA-1 eukaryotic expression vector was transfected into HepG 2.2.15 hepatoma cell line with lipofectamine. HBV e antigen and s antigen was checked with ELISA method. RT-PCR was used to identify expression of some transcription factors that involved in HBV transcription such as HNF1

HNF2

HNF3

HNF4

RXRα

PPARα

and C/EBP. Results Compared with normal HepG 2.2.15 hepatoma cell

the expression of e antigen was obviously high and s antigen was depressed in the HepG 2.2.15 hepatoma cell transfected with pcDNA3-TIA-1 vector. Compared with normal HepG 2.2.15 hepatoma cell

the mRNA level of HNF1

HNF2

RXRα changed significantly. Conclusion These data show that TIA-1 may interact with liver-enriched transcription factors and regulate HBV e antigen and s antigen expression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Up-regulation of stathmin by specialized protein 1 promotes proliferation and inhibits apoptosis in uterine leiomyoma cells

Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma

Remodeling characteristics of H3K27me3-marked silencer in gastric intestinal metaplasia and its transcriptional regulatory mechanism

Reprogramming characteristics of H3K27ac-marked enhancer in gastric intestinal metaplasia and its transcriptional regulatory mechanism

MAPK signal pathway regulates Fas ligand expression in human cholangiocarcinoma cells and its mechanism

Related Author

Caihong XU

Shanshan LIU

Peifang CHEN

Shengqing PAN

Fang FANG

Jiali LIU

Beibei XUAN

Quan QUAN

Related Institution

Department of Gynaecology and Obstetrics, Nanjing Tongren Hospital, School of Medicine of Southeast University

Department of Critical Care Medicine, Nanjing Tongren Hospital, School of Medicine of Southeast University

Department of Obstetrics, the First Affiliated Hospital of Chongqing Medical University

⁰