Evaluation and quantification of total antioxidative capability of fruit by microassay

唐勇; 赵靖; 徐静; 张婷; 张彦奇; 糜漫天

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Evaluation and quantification of total antioxidative capability of fruit by microassay

更新时间：2026-01-09

- Evaluation and quantification of total antioxidative capability of fruit by microassay
- Journal of Army Medical University Vol. 30, Issue 6, Pages: 517-520(2008)
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  CLC： R151.2;TS201.2
- Published：2008
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唐勇,赵靖,徐静,张婷,张彦奇,糜漫天.植物总抗氧化能力的微孔板定量测定及评价[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):517-520.

　TANG Yong,ZHAO Jing,XU Jing,ZHANG Ting,ZHANG Yan-qi,MI Man-tian.Evaluation and quantification of total antioxidative capability of fruit by microassay[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):517-520.
唐勇,赵靖,徐静,张婷,张彦奇,糜漫天.植物总抗氧化能力的微孔板定量测定及评价[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):517-520. DOI：

　TANG Yong,ZHAO Jing,XU Jing,ZHANG Ting,ZHANG Yan-qi,MI Man-tian.Evaluation and quantification of total antioxidative capability of fruit by microassay[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):517-520. DOI：

摘要

目的结合动力学曲线分析评价植物中常见的天然抗氧化物对2

2-二苯基苦基苯肼自由基（DPPH·）的清除能力和反应动力学特点。方法光谱扫描分析DPPH·的吸收特性；采用96微孔板结合酶标仪的微孔板定量法（microplate quantification

MQ）分析测定维生素C、绿原酸、药食两用木瓜、橙子、芒果和樱桃对DPPH·的清除能力和动力学曲线，以单位质量（g）或体积（ml）的样品清除DPPH·的量（μg）为总抗氧化能力值（total antioxidative capability，TAC）表示。结果 DPPH·在酶标仪的490 nm检定波长上有显著吸收峰；维生素C和绿原酸纯品验证了MQ对复合抗氧化物清除DPPH·效能的加合性；根据各动力学曲线特征确定了不同果汁在清除DPPH·反应达到稳定态时的最大TAC（μg DPPH·/ml）：木瓜汁87.23（P0.05），芒果汁62.556（P0.05），甜橙汁 58.778（P0.05），樱桃汁 49.072（P0.05）。结论采用MQ测定方法，比传统经典方法试剂耗量小、批次处理量大，以及能同时分析评价多样品之间清除DPPH·的差异特性，为评价比较不同植物的总抗氧化能力提供了更快捷的方法。

Abstract

Objective 〓To investigate the different kinetics and capability of natural antioxidants scavenging free radical 2

2-diphenyl-1-picrylhydrazyl (DPPH·). Methods Spectrum scanning was used to analyze the characteristic absorbance of DPPH. Microplate quantification of DPPH· was used to quantify total antioxidative capability (TAC) of vitamin C

chlorogenic acid

Chaenomeles

citrus

mango and cherry. TAC represents the amount (μg) of DPPH· scavenged by each gram or each milliliter of the samples. Results The highest absorbance of DPPH· was shown on microplate reader at wavelength of 490 nm. Vitamin C and chlorogenic acid verified good correlation between antioxidants concentration and amount of scavenging DPPH· by microplate quantification assay. The TAC of the fruit juices was as follows: Chaenomeles 87.23 (P0.05)

mango 62.556 (P0.05)

citrus 58.778 (P0.05) and cherry 49.072 (P0.05). Conclusion Microplate quantification assay reveals the characteristics of nature scavengers to complex compounds. It has the advantages of less reagents used

high throughput

easy accessibility to kinetic curve and minimized experimental error.

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