Construction of eukaryotic expression vector of human HYAL1 gene and its expression in MCF-7 and ZR-75-30 cells

苏新良; 任国胜; 王小毅; 谭金祥

您当前的位置：

首页 >

文章列表页 >

Construction of eukaryotic expression vector of human HYAL1 gene and its expression in MCF-7 and ZR-75-30 cells

更新时间：2026-01-09

- Construction of eukaryotic expression vector of human HYAL1 gene and its expression in MCF-7 and ZR-75-30 cells
- Journal of Army Medical University Vol. 30, Issue 2, Pages: 146-149(2008)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394-33;R394.2;R737.9
- Published：2008
- 稿件说明：
移动端阅览
苏新良,任国胜,王小毅,谭金祥.人乳腺癌细胞HYAL1基因真核表达载体的构建及其在MCF-7和ZR-75-30细胞中的表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):146-149.

　SU Xin-liang,REN Guo-sheng,WANG Xiao-yi,TAN Jin-xiang.Construction of eukaryotic expression vector of human HYAL1 gene and its expression in MCF-7 and ZR-75-30 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):146-149.
苏新良,任国胜,王小毅,谭金祥.人乳腺癌细胞HYAL1基因真核表达载体的构建及其在MCF-7和ZR-75-30细胞中的表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):146-149. DOI：

　SU Xin-liang,REN Guo-sheng,WANG Xiao-yi,TAN Jin-xiang.Construction of eukaryotic expression vector of human HYAL1 gene and its expression in MCF-7 and ZR-75-30 cells[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):146-149. DOI：

摘要

目的构建人乳腺癌HYAL1基因的真核表达载体并稳定转染HYAL1基因低表达的乳腺癌MCF-7和ZR-75-30细胞株，为进一步探讨HYAL1基因在乳腺癌的侵袭和转移中的作用奠定基础。方法采用RT-PCR技术从高表达HYAL1的人乳腺癌细胞株MDA-MB-435S中扩增透明质酸酶HYLAL1基因

并将其插入真核表达载体pcDNA3.1/V5-His-TOPO中，构建的重组表达质粒经脂质体介导转染HYAL1基因低表达的乳腺癌MCF-7和ZR-75-30细胞。结果用RTPCR成功地扩增出1条1 332 bp的DNA片段，经限制性内切酶酶切分析和DNA测序证明已经成功构建了人乳腺癌HYAL1基因的真核表达载体。RT-PCR证明转染了重组质粒的乳腺癌MCF-7和ZR-75-30细胞可以高效稳定的表达HYAL1基因，且其穿透细胞外基质的能力增强。结论成功构建了人乳腺癌HYAL1基因的真核表达载体，获得了稳定表达人HYAL1基因的MCF-7和ZR-75-30细胞克隆，且其侵袭能力增强。

Abstract

Objective To construct the eukaryotic expression vector of human HYAL1 gene and obtain MCF-7 and ZR-75-30 cell clones expressing HYAL1 gene stably. Methods The cDNA encoding HYAL1 gene of human breast cancer was amplified by RT-PCR from the total RNA isolated from human MDA-MB-435S cells and inserted into pcDNA3.1/V5-His-TOPO vector. The recombinant plasmid was transferred into MCF-7 and ZR-75-30 cells. Results A 1332-bp DNA fragment was successfully amplified from human MDA-MB-435S cell. Restriction enzyme digestion analysis and DNA sequencing showed that HYAL1 gene was inserted into recombinant vector. RT-PCR analysis revealed that HYAL1 gene could be expressed stably in the transfected MCF-7 and ZR-75-30 and it had strong invasive potential. Conclusion The eukaryotic expression vector of human HYAL1 gene was successfully constructed. MCF-7 and ZR-75-30 cell clones that can express HYAL1 gene were obtained and can promote the invasion.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of HYAL1 gene overexpression on invasive, angiogenic and proliferative ability of breast cancer cells

Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis

Expression of miR-616 in osteosarcoma and its role in proliferation, apoptosis, migration and invasion of tumor cells

Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

Effect of calumenin on metastasis and invasion of gastric cancer and prognosis of patients

Related Author

苏新良

任国胜

王小毅

谭金祥

LI Na

LI Hao

LIN Kun

HE Yanxin

Related Institution

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

⁰