Objective  To explore the preservation effect of a modified platelets (PLT) additive solution (PAS-ⅢM) as a substitute for plasma in the storage of platelet concentrates at low temperature.   Methods  Apheresis platelets from 6 donors were divided and stored in different media

that is

A:70%PAS-ⅢM/30%plasma stored at 10 ℃

B: plasma stored at 22 ℃

C: plasma/trehalose stored at -85 ℃. The samples of in group A and B were respectively collected in 0

1

5

7 and 9 days after storage

and those of group C on day 20 after storage. Platelet count(PLT)

mean platelet volume(MPV)

platelet distributing width(PDW)

CD62p

hypotonic shock response(HSR)

platelet aggregation (PAgT)

pH

lactic dehydrogenase (LDH)

glucose consumption were evaluated.   Results  The expression of CD62p in group A and B group were increased in a time-dependent manner

but HSR and PAgT were decreased. CD62p expression

LDH release

glucose consumption were significant higher

and HSR

PAgT were significant lower in group B than in group A (P0.05). After 5 days’ storage

pH value in group B were significant decreased (P0.05). LDH release were significant higher in samples of C group and the expression of CD62p were lower than those of other 2 groups (P0.05). PDW of C group were increased after storage and significant higher than that of other 2 groups(besides that of B group on day 5) (P0.05). In all storage groups

PLT had no significant difference.   Conclusion  PAS-ⅢM could be a good substitute for plasma in platelet storage

and can protect the platelets function at low temperature.