In vitro and in vivo biological function of p48--a new type of lipopolysaccharide binding protein

葛晓冬; 杨艳丽; 段光杰; 陈锐; 潘峰; 朱江; 刘友生

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In vitro and in vivo biological function of p48--a new type of lipopolysaccharide binding protein

更新时间：2026-01-09

- In vitro and in vivo biological function of p48--a new type of lipopolysaccharide binding protein
- Journal of Army Medical University Vol. 30, Issue 2, Pages: 142-145(2008)
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  CLC： R341;R392-33;R392.12
- Published：2008
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葛晓冬,杨艳丽,段光杰,陈锐,潘峰,朱江,刘友生.内毒素结合蛋白样分子p48体内外生物学活性的初步研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):142-145.

　GE Xiao-dong,YANG Yan-li,DUAN Guang-jie,CHEN Rui,PAN Feng,ZHU Jiang,LIU You-sheng.In vitro and in vivo biological function of p48--a new type of lipopolysaccharide binding protein[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):142-145.
葛晓冬,杨艳丽,段光杰,陈锐,潘峰,朱江,刘友生.内毒素结合蛋白样分子p48体内外生物学活性的初步研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):142-145. DOI：

　GE Xiao-dong,YANG Yan-li,DUAN Guang-jie,CHEN Rui,PAN Feng,ZHU Jiang,LIU You-sheng.In vitro and in vivo biological function of p48--a new type of lipopolysaccharide binding protein[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):142-145. DOI：

摘要

目的以LBP为对照，对比研究p48的体内、外生物学功能。方法采用生物传感器、流式细胞分析技术研究p48与LPS及Lipid A的结合力。通过体外培养人单核细胞（U937）和小鼠体内TNF-α的分泌实验，研究p48的生物学功能。采用ELISA方法初步探索p48与LBP是否具有一定相似的空间结构。结果 p48能促进LPS与PBMC结合，其活性为LBP的81％。p48能与LPS及Lipid A结合，其亲和常数（KD）分别为1.59×10

-6

、1.48×10

-6

mol/L。体内外实验中p48均能促进TNF-α的分泌，其生物学活性约为LBP的77％。高浓度抗NH-LBP Fab抗体能与p48特异性结合，p48部分空间结构与LBP可能有一定的相似性。结论 p48具有与LBP相近似的生物学功能，在机体炎症反应中也发挥较重要的作用。

Abstract

Objective To study the biological function of p48 by comparing with that of lipopolysaccharide binding protein (LBP). Methods The ability of p48 to bind lipopolysaccharide (LPS) or lipid A was determined by the experiment of Biosensor and flow cytometry. The biological function of p48 was identified by measuring the secretion of tumor necrosis factor alpha (TNF-α) in human monocyte (U937) or mice. Using the monoclonal antibody (mAb) Fab to NH-LBP

the spatial structure of p48 was assessed by ELISA to determine whether its structure is similar to that of LBP. Results p48 could accelerate the binding of LPS with peripheral blood mononuclear cells (PBMC)

whos

e ability is about 81% of LBP. p48 could bind LPS or lipid A directly

with the affinity constant of 1.59×10

-6

mol/L or 1.48×10

-6

mol/L respectively. The secretion rate of TNF-α was enhanced by p48

whose effect is about 77% of LBP in vivo or in vitro. That the mAb Fab could bind p48 at high concentration indicated some similar spatial structure between p48 and LBP. Conclusion p48

similar to LBP in biological function

plays an important role in innate immunity and inflammatory reaction.

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