Establishment of mouse model of premature ovarian failure

李彩霞; 王凤英; 李玉艳; 俞炽阳

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Establishment of mouse model of premature ovarian failure

更新时间：2026-01-09

- Establishment of mouse model of premature ovarian failure
- Journal of Army Medical University Vol. 30, Issue 6, Pages: 506-509(2008)
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  CLC： R-332;R711.75
- Published：2008
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李彩霞,王凤英,李玉艳,俞炽阳.小鼠卵巢早衰动物模型的构建[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):506-509.

　LI Cai-xia,WANG Feng-ying,LI Yu-yan,YU Chi-yang.Establishment of mouse model of premature ovarian failure[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):506-509.
李彩霞,王凤英,李玉艳,俞炽阳.小鼠卵巢早衰动物模型的构建[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):506-509. DOI：

　LI Cai-xia,WANG Feng-ying,LI Yu-yan,YU Chi-yang.Establishment of mouse model of premature ovarian failure[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):506-509. DOI：

摘要

目的探讨用化学药物及放射性处理，建立小鼠卵巢早衰动物模型的方法。方法将80只昆明小鼠分为化学药物处理组、放射组、对照组、去势组。化学药物组使用环磷酰胺和白消安干预，放射组采用

Coγ射线放射。通过观察阴道脱落细胞监测小鼠动情周期变化、雌激素水平、卵巢及子宫内膜组织学改变等指标，判断建模效果。结果化学药物组、放射组与对照组相比动情期构成比降低、动情间期构成比增加(P0.01)；雌激素水平明显降低(P0.01)，接近去势组水平(P0.05)；两个处理组小鼠均可见卵巢萎缩、生长卵泡数明显减少、间质纤维化；卵巢未闭锁原始卵泡数显著减少(P0.01)，且随建模后时间的延长而呈进一步下降趋势，但化学药物组与放射组各相同时间段之间无明显差异。结论以化学药物及放射方法能够成功建立小鼠卵巢早衰模型。

Abstract

Objective To develop a mouse model of premature ovarian failure (POF) by chemical treatment or radiotreatment. Methods Eighty Kunming mice were divided into four groups

including chemical treatment group that were administered Cyclophosphamide and Busulfan

radiotreatment group receiving

Co γ ray irradiation

control group and castrated group. To evaluate the animal model

vaginal smears were collected daily for estrous cycle determination

and serum hormone levels and histological changes in ovaries were examined. Results Estrous cycles became irregular after chemical treatment or radiotreatment (P0.01). The serum estrogen levels of chemical treatment group and radiotreatment group were markedly lower than those of control group (

P0.01)

but close to those of castrated group (P0.05). Compared to control group

ovaries from chemical treatment and radiotreatment groups showed ovarian atrophy and interstitial fibrosis. The number of growing follicles and nonatretic primordial follicles was notably reduced (P0.01) in an increasingly descending way

but showed no significant difference between chemical treatment group and radiotreatment group. Conclusion The mouse model of POF is successfully established by applying experimental irradiation of

Co γ ray or injection of Cyclophosphamide and Busulfan.

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