Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ<sub>1-40</sub> injuried rats

李志方; 唐军; 李露斯; 杨丽; 阴金波; 徐海伟

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Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ_1-40 injuried rats

更新时间：2026-01-09

- Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ_1-40 injuried rats
- Journal of Army Medical University Vol. 30, Issue 7, Pages: 595-599(2008)
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  CLC： R329.2;R338.64;R749.1
- Published：2008
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李志方,唐军,李露斯,杨丽,阴金波,徐海伟.C17.2神经干细胞移植Aβ1-40损伤大鼠海马后的分化及对学习记忆的改善[J].陆军军医大学学报(原第三军医大学学报),2008,30(07):595-599.

　LI Zhi-fang,TANG Jun,LI Lu-si,YANG Li,YIN Jin-bo,XU Haiwei.Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ1-40 injuried rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(07):595-599.
李志方,唐军,李露斯,杨丽,阴金波,徐海伟.C17.2神经干细胞移植Aβ1-40损伤大鼠海马后的分化及对学习记忆的改善[J].陆军军医大学学报(原第三军医大学学报),2008,30(07):595-599. DOI：

　LI Zhi-fang,TANG Jun,LI Lu-si,YANG Li,YIN Jin-bo,XU Haiwei.Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ1-40 injuried rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(07):595-599. DOI：

摘要

目的探讨永生化神经干细胞株C17.2移植Aβ

1-40

损伤大鼠海马后的存活、分化以及细胞移植对AD大鼠的治疗作用。方法单侧海马齿回注射凝聚态Aβ

1-40

复制AD大鼠模型；C17.2神经干细胞移植到海马Aβ

1-40

注射处，免疫荧光观察移植细胞的存活、分化，Morris水迷宫检测干细胞移植对AD大鼠空间学习记忆障碍的改善情况。结果 C17.2神经干细胞移植到AD大鼠海马后存活良好，DAPI核染显示移植细胞广泛迁移，Cy3荧光显示大部分移植细胞分化为GFAP阳性细胞，并可见一定数量的移植细胞分化为NF200阳性细胞，其中部分发出类似神经元的长突起。行为学检测表明干细胞移植对AD大鼠空间学习记忆能力有明显改善。结论永生化神经干细胞移植AD大鼠海马后能有效存活，移植细胞可分化为胶质细胞、神经元并能改善AD大鼠学习记忆功能障碍。

Abstract

Objective To explore the differentiation and therapeutic effects of immortalized C17.2 neural stem cells in the rat model of Alzheimer’s disease (AD). Method Pre-aggregated Aβ

1-40

was microinjected into the dentate gyrus of rat hippocampus to mimic AD

and C17.2 neural stem cells were transplanted into the vicinity of Aβ

1-40

2 weeks later. The survival and differentiation of grafted cells were observed with immunofluorescence staining. Learning and memory abilities of animals were evaluated by Morris water maze test. Results The grafted cells survived and migrated exte

nsively. Most C17.2 cells differentiated into GFAP-positive glia and some into NF200-positive neurons with long processes into the host brain. Learning and memory of AD rats were significantly improved after C17.2 neural stem cell transplantation. Conclusion The satisfactory survival

differentiation and improvement on learning and memory indicate the therapeutic potential of immortalized neural stem cells for neuronal replacement.

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Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ1-40 injuried rats

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摘要

Abstract

关键词

Keywords

references

Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ_1-40 injuried rats