Differential proteomic analysis of HeLa cells induced by TRAIL

赖国旗; 邱宗荫; 向廷秀; 宋敏; 胡小龙; 何明忠; 谭冬梅

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Differential proteomic analysis of HeLa cells induced by TRAIL

更新时间：2026-01-09

- Differential proteomic analysis of HeLa cells induced by TRAIL
- Journal of Army Medical University Vol. 30, Issue 4, Pages: 318-321(2008)
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  CLC： R394-33;R73-362;R737.33
- Published：2008
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赖国旗,邱宗荫,向廷秀,宋敏,胡小龙,何明忠,谭冬梅.肿瘤坏死因子相关凋亡诱导配体诱导HeLa细胞凋亡的差异蛋白质组分析[J].陆军军医大学学报(原第三军医大学学报),2008,30(04):318-321.

　LAI Guo-qi,QIU Zong-yin,XIANG Ting-xiu,SONG Min,HU Xiao-long,HE Ming-zhong,TAN Dong-mei.Differential proteomic analysis of HeLa cells induced by TRAIL[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(04):318-321.
赖国旗,邱宗荫,向廷秀,宋敏,胡小龙,何明忠,谭冬梅.肿瘤坏死因子相关凋亡诱导配体诱导HeLa细胞凋亡的差异蛋白质组分析[J].陆军军医大学学报(原第三军医大学学报),2008,30(04):318-321. DOI：

　LAI Guo-qi,QIU Zong-yin,XIANG Ting-xiu,SONG Min,HU Xiao-long,HE Ming-zhong,TAN Dong-mei.Differential proteomic analysis of HeLa cells induced by TRAIL[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(04):318-321. DOI：

摘要

目的比较分析肿瘤坏死因子相关凋亡诱导配体（TNF-related apoptosis-inducing ligand， TRAIL）诱导人宫颈癌细胞系HeLa细胞凋亡前后蛋白质组中的差异表达蛋白，以进一步了解TRAIL的作用机制。方法以TRAIL诱导人宫颈癌细胞系HeLa细胞凋亡为模型，用双向电泳技术分离HeLa全细胞蛋白质，MALDI-TOF质谱鉴定，MS-FIit数据库分析，RT-PCR半定量方法检测JNK2的表达。结果鉴定得到12个明显的差异蛋白点，其中8个蛋白点上调，4个蛋白点下调。JNK2的表达与TRAIL有良好的量效关系。结论 JNKK、MAPKAPK-2、Dip13 beta、SHPS-1、DDBb、JEM-1、Macoilin、Leucine-rich repeat-containing protein 14、Ena/VASP-like protein、PTPase-MEG2、GRB10 adaptor protein、THUMP domain-containing protein 1为TRAIL诱导HeLa细胞凋亡的新的相关蛋白。

Abstract

Objective To detect the differential proteins between HeLa cells treated and untreated by TRAIL so as to further understand the mechanisms of apoptosis induced by TRAIL in HeLa cells. Methods The differential proteins were observed by 2D electrophoresis

identified by matrixassisted laser desorption/ionizationtime of flightmass spectrometry (MALDI-TOF-MS) and analyzed by MS Fit. The expression of JNK2 was examined by semiquantitative RT-PCR method. Results There were twelve differential proteins between HeLa cells treated and untreated by TRAIL

8 up-regulated and 4 down-regulated. There was a good dose-effect relationship between JNK2 and TRAIL. Conclusion JNKK

MAPKAPK-2

Dip13 beta

SHPS-1

DDBb

JEM-1

Macoilin

Leucine-rich repeat-containing protein 14

Ena/VASP-like protein

PTPase-MEG2

GRB10 adaptor protein

THUMP domain-containing protein 1 take part in the apoptosis of HeLa cells induced by TRAIL.

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