Microinjection of Muscimol into cerebellular dentate-interpositus nuclei affects acquisition of classical eyeblink conditioning in guinea pigs

林曦; 胡波; 杨丽; 隋建峰

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Microinjection of Muscimol into cerebellular dentate-interpositus nuclei affects acquisition of classical eyeblink conditioning in guinea pigs

更新时间：2026-01-09

- Microinjection of Muscimol into cerebellular dentate-interpositus nuclei affects acquisition of classical eyeblink conditioning in guinea pigs
- Journal of Army Medical University Vol. 30, Issue 6, Pages: 499-502(2008)
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  CLC： R-33;R338.23;R338.61
- Published：2008
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林曦,胡波,杨丽,隋建峰.蝇蕈醇小脑齿状-中位核微注射对豚鼠经典眨眼条件反射建立的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):499-502.

　LIN Xi,HU Bo,YANG Li,SUI Jian-feng.Microinjection of Muscimol into cerebellular dentate-interpositus nuclei affects acquisition of classical eyeblink conditioning in guinea pigs[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):499-502.
林曦,胡波,杨丽,隋建峰.蝇蕈醇小脑齿状-中位核微注射对豚鼠经典眨眼条件反射建立的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):499-502. DOI：

　LIN Xi,HU Bo,YANG Li,SUI Jian-feng.Microinjection of Muscimol into cerebellular dentate-interpositus nuclei affects acquisition of classical eyeblink conditioning in guinea pigs[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):499-502. DOI：

摘要

目的观察小脑齿状-中位核在豚鼠经典眨眼条件反射建立过程中的作用。方法采用500 ms正弦波纯音和100 ms束状氧气流的配对刺激，训练豚鼠建立经典眨眼条件反射。在条件反射训练的第4~6天，向豚鼠双侧小脑齿状-中位核微注射蝇蕈醇。整个实验过程中，使用高分辨率的张力换能器检测豚鼠的眨眼反应。结果 ①蝇蕈醇注射组豚鼠训练第4~6天的条件反应率均显著低于第3训练日水平(P

=0.005，P

=0.004，P

=0.010)；②蝇蕈醇注射组豚鼠训练第7、8天条件反应率仅分别相当于对照组豚鼠第4、5训练日水平(P

=0.061，P

=0.669)；③在此过程中，蝇蕈醇注射组豚鼠非条件眨眼反应的幅度和峰潜伏期均无显著变化(P

=0.926，P

=0.939)。结论小脑齿状-中位核是运动性学习神经回路的重要组成部分，控制着眨眼条件反应的获得和表达。

Abstract

Objective To investigate the role of cerebellar dentateinterpositus (D-I) nuclei during acquisition of classical eyeblink conditioning in guinea pigs. Methods A 500 ms tone conditioned stimulus (CS) was paired with a 100 ms corneal oxygen-puff unconditioned stimulus (US) in a delay paradigm. Guinea pigs were trained daily to acquire the classically conditioned eyeblink responses. Micorinjections of Muscimol into bilateral cerebellar D-I nuclei were performed during the 4-6 day of training session. A high-resolution potentiometer was used to detect t

he eyeblink responses. Results The conditioned response (CR) rates of Muscimolinjected group on session 4-6 were significantly lower than their rates on session 3 (P

=0.005， P

=0.004， P

=0.010); Their rates on session 7 and 8 were as the same level as those without microinjection on session 4 and 5 (P

=0.061， P

=0.669). However

there was no significant difference on their amplitude and peak latency of unconditioned responses (URs) during the entire course of training (P

=0.926， P

=0.939). Conclusion The cerebellar D-I nucleus is actively involved in the neural circuitry of motor learning

which is essential for both acquisition and performance of the classically conditioned eyeblink responses.

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