Hypoxia induced non-concordant expression of gastrocnemius mitochondrial electron transport chain complexes in Wistar rats

高文祥; 黄缄; 高钰琪; 谭小玲; 刘福玉; 蔡明春; 陈建

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Hypoxia induced non-concordant expression of gastrocnemius mitochondrial electron transport chain complexes in Wistar rats

更新时间：2026-01-09

- Hypoxia induced non-concordant expression of gastrocnemius mitochondrial electron transport chain complexes in Wistar rats
- Journal of Army Medical University Vol. 30, Issue 3, Pages: 223-225(2008)
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  CLC： R322.74;R329.25;R364.4
- Published：2008
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高文祥,黄缄,高钰琪,谭小玲,刘福玉,蔡明春,陈建.低氧调节大鼠骨骼肌线粒体呼吸链复合体非协同性表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):223-225.

　GAO Wen-xiang,HUANG Jian,GAO Yu-qi,TAN Xiao-ling,LIU Fu-yu,CAI Ming-chun,CHEN Jian.Hypoxia induced non-concordant expression of gastrocnemius mitochondrial electron transport chain complexes in Wistar rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):223-225.
高文祥,黄缄,高钰琪,谭小玲,刘福玉,蔡明春,陈建.低氧调节大鼠骨骼肌线粒体呼吸链复合体非协同性表达[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):223-225. DOI：

　GAO Wen-xiang,HUANG Jian,GAO Yu-qi,TAN Xiao-ling,LIU Fu-yu,CAI Ming-chun,CHEN Jian.Hypoxia induced non-concordant expression of gastrocnemius mitochondrial electron transport chain complexes in Wistar rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):223-225. DOI：

摘要

目的观察慢性低氧时大鼠线粒体呼吸链复合体Ⅰ、Ⅱ、Ⅲ、Ⅴ蛋白表达变化并讨论其病理生理学意义。方法成年雄性Wistar大鼠随机分为慢性低氧(4 500 m

30 d)组和对照组，取双侧腓肠肌，分离线粒体，用Clark氧电极法检测线粒体Ⅲ态呼吸(state 3

ST3)、Ⅳ态呼吸(state 4

ST4)和呼吸控制率(respiratory control ratio

RCR)，用Western blot检测线粒体呼吸链复合体Ⅰ、Ⅱ、Ⅲ、Ⅴ蛋白的表达。结果慢性低氧组大鼠腓肠肌线粒体ST3和RCR显著低于对照组(P0.05)。慢性低氧组大鼠腓肠肌线粒体复合体Ⅰ 30×10

亚基、复合体Ⅱ 70×10

亚基、复合体Ⅴ α亚基蛋白表达量显著低对照组(P0.05)。2组线粒体ST4和复合体Ⅲ核心亚基2蛋白表达无显著性差异(P0.05)。结论〖HTSS〗低氧可调节大鼠骨骼机线粒体复合体蛋白非协同性表达，导致线粒体氧化磷酸化功能下降。

Abstract

Objective To explore the expression changes of mitochondrial electron transport chain (ETC) complexes Ⅰ，Ⅱ，Ⅲ and Ⅴ induced by chronic hypoxia. Methods Sixteen adult male Wistar rats were randomly and equally divided into hypoxic group (4 500 m

30 d) and the normoxic control. Hibateral gastrocnemii were collected and mitochondria were isolated. Respiratory state 3 (ST3)

state 4 (ST4) and respiratory control rate (RCR) were measured with Clark oxygen electrode. Mitochondrial ETC complexes Ⅰ，Ⅱ，Ⅲ and Ⅴ expressions were measured with Western blot. Results ST3 and RCR were significantly lower in hypoxic group than in the control. Mitochondrial ETC complexes Ⅰ，Ⅱ，Ⅲ and Ⅴ expressions were significantly lower in hypoxic group than in the control. No significant difference of ST4 and complex Ⅲ core subunit 2 was found between the 2 groups. Conclusion Non-concordant expression of gastrocnemius mitochondrial ETC complexes can be induced by chronic hypoxia

resulting in the decrease of mitochondrial oxidative phosphorylation function.

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