SOCS3 gene inhibits the growth of A549 cell transplantation tumor in nude mice

余祖滨; 白莉; 姚珂; 闵家新; 张国强; 肖颖彬

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SOCS3 gene inhibits the growth of A549 cell transplantation tumor in nude mice

更新时间：2026-01-09

- SOCS3 gene inhibits the growth of A549 cell transplantation tumor in nude mice
- Journal of Army Medical University Vol. 30, Issue 2, Pages: 127-129(2008)
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  CLC： R394.3;R73-35;R734.2
- Published：2008
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余祖滨,白莉,姚珂,闵家新,张国强,肖颖彬.SOCS3基因转染对人肺腺癌细胞株A549裸鼠移植瘤生长的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):127-129.

　YU Zu-bin,BAI Li,YAO Ke,MIN Jia-xin,ZHANG Guo-qiang,XIAO Ying-bin.SOCS3 gene inhibits the growth of A549 cell transplantation tumor in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):127-129.
余祖滨,白莉,姚珂,闵家新,张国强,肖颖彬.SOCS3基因转染对人肺腺癌细胞株A549裸鼠移植瘤生长的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):127-129. DOI：

　YU Zu-bin,BAI Li,YAO Ke,MIN Jia-xin,ZHANG Guo-qiang,XIAO Ying-bin.SOCS3 gene inhibits the growth of A549 cell transplantation tumor in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):127-129. DOI：

摘要

目的观察细胞因子信号负调控因子3（suppressors of cytokine signaling 3， SOCS3）对人肺腺癌细胞株A549裸鼠移植瘤生长的影响。方法将A549细胞、稳定转染空载体的A549细胞、稳定转染SOCS3载体的A549细胞分别接种至裸鼠皮下，复制A549裸鼠皮下移植瘤模型，观察各组细胞在裸鼠体内的成瘤率，测量肿瘤体积、瘤质量并绘制肿瘤生长曲线；免疫组织化学法和Western blot法检测肿瘤组织中信号转导和转录活化因子3（signal transduction and activators of transcription 3， STAT3)酪氨酸磷酸化水平。结果与未转染组、空质粒转染组相比，SOCS3稳定表达组细胞裸鼠移植瘤生长缓慢，细胞成瘤数、瘤体体积和瘤质量显著降低（P0.01）；瘤组织标本中酪氨酸磷酸化STAT3水平显著降低（P0.01）。结论 SOCS3能显著抑制A549细胞在体内的生长能力，其机制可能与下调细胞内STAT3活性水平有关。

Abstract

Objective To explore the effect of transfected SOCS3 gene on the growth of A549 cells in vivo. Methods Nude mice were transplanted subcutaneously with A549 cells

A549 cells stably transfected with SOCS3

A549 transfected with empty vectors

respectively. The tumor volume and weight were measured. Immunohistochemistry and Western blot were used to detect the level of tyrosinephosphorylated protein STAT3 in tumor tissue. Results Compared with A549 cells and A549 transfected with empty vectors

A549 cells stably transfected with SOCS3 showed lower tumorigenicity and grew slowly in tumor volume and weight

as well as lower tyrosine-phosphorylated STAT3 level in tumor tissue (all P0.01). Conclusion SOCS3 inhibits the growth of A549 cells by lowering the tyrosine-phosphorylated protein STAT3 level in vivo.

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