Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549

余祖滨; 白莉; 姚珂; 张国强; 闵家新; 肖颖彬

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Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549

更新时间：2026-01-09

- Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549
- Journal of Army Medical University Vol. 30, Issue 7, Pages: 588-591(2008)
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  CLC： R394.2;R730.23;R734.2
- Published：2008
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余祖滨,白莉,姚珂,张国强,闵家新,肖颖彬.SOCS3对肺腺癌细胞株A549迁移和侵袭能力的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(07):588-591.

　YU Zu-bin,BAI Li,YAO Ke,ZHANG Guo-qiang,MIN Jia-xin,XIAO Ying-bin.Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(07):588-591.
余祖滨,白莉,姚珂,张国强,闵家新,肖颖彬.SOCS3对肺腺癌细胞株A549迁移和侵袭能力的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(07):588-591. DOI：

　YU Zu-bin,BAI Li,YAO Ke,ZHANG Guo-qiang,MIN Jia-xin,XIAO Ying-bin.Effects of SOCS3 gene on migration and invasion of lung adenocarcinoma cell line A549[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(07):588-591. DOI：

摘要

目的观察细胞因子信号负调控因子3（suppressors of cytokine signaling

SOCS3）对人肺腺癌细胞株A549迁移和侵袭能力的影响，并初步探讨其机制。方法建立SOCS3稳定表达细胞株；Boyden Chamber分析法测定细胞的迁移和侵袭能力；半定量RT-PCR检测细胞基质金属蛋白酶（matrix metalloproteinases

MMPs）2

9 mRNA表达；明胶酶谱分析法测定MMP2

9蛋白活性水平。结果 SOCS3稳定表达株细胞迁移和侵袭能力显著下降 (P0.05)；半定量RT-PCR和明胶酶谱分析结果显示SOCS3可显著抑制MMP2

9表达和活性 (P0.05)。结论 SOCS3能通过抑制MMP2

9表达和活性明显降低A549的体外迁移和侵袭能力。

Abstract

Objectiv To explore the mechanism of SOCS3 on the migration and invasion of human lung adenocarcinoma cell line A549. Methods A549 cells that express SOCS3 stably were generated. The migration and invasion abilities of A549 cells were observed by Boyden Chamber assay. The mRNA and protein expressions of MMP-2 and MMP-9 were assessed by semi-quantitive RT-PCR and gelatin zymography respectively. Results Compared with the control group

the migration and invasion abilities of A549 cells with stable SOCS3 expression were significantly lower (P0.05). The mRNA expression and activities of MMP-2 and MMP-9 in A549 cells with stable SOCS3 expression were inhibited significantly (P0.05). Conclusion SOCS3 protein inhibits the migration and invasion abilities of A549 cells probably by down-regulating the expression and activation of MMP-2 and MMP-9.

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