Nanobacteria: isolation from prostatic calculus and identification of its 16S rRNA gene

沈学成; 杨杰; 饶贤才; 宋波; 周占松

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Nanobacteria: isolation from prostatic calculus and identification of its 16S rRNA gene

更新时间：2026-01-09

- Nanobacteria: isolation from prostatic calculus and identification of its 16S rRNA gene
- Journal of Army Medical University Vol. 30, Issue 2, Pages: 124-126(2008)
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  CLC： R372;R394;R697.340.2
- Published：2008
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沈学成,杨杰,饶贤才,宋波,周占松.前列腺结石患者结石中纳米细菌的分离和16S rRNA基因的鉴定[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):124-126.

　SHEN Xue-cheng,YANG Jie,RAO Xian-cai,SONG Bo,ZHOU Zhan-song.Nanobacteria: isolation from prostatic calculus and identification of its 16S rRNA gene[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):124-126.
沈学成,杨杰,饶贤才,宋波,周占松.前列腺结石患者结石中纳米细菌的分离和16S rRNA基因的鉴定[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):124-126. DOI：

　SHEN Xue-cheng,YANG Jie,RAO Xian-cai,SONG Bo,ZHOU Zhan-song.Nanobacteria: isolation from prostatic calculus and identification of its 16S rRNA gene[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):124-126. DOI：

摘要

目的检测纳米细菌在前列腺结石中的分布情况

探讨其在前列腺结石发病中的作用。方法采集前列腺结石患者的前列腺结石标本

分离培养纳米细菌，采用PCR法对纳米细菌16S rRNA基因进行扩增，电泳分析结果并测序。结果将PCR得到的特异性片段的测序结果与已知纳米细菌16S rRNA序列进行比较，相似度为98%：分值=2 480 bits (1 290)

期望值=0.0；相似度=1 387/1 409 (98%)

缺失=4/1 409 (0%)；链=正/正。结论 PCR法和测序可以作为纳米细菌16S rRNA基因的检测手段；前列腺结石患者的结石中存在着纳米细菌的感染。

Abstract

Objective To explore the distribution of nanobacteria in prostatic calculus and investigate its role in the formation of prostatic calculus. Methods The stones of 40 patients with prostatic calculus was used to isolate and culture the possible bacteria. The genomes of obtained bacteria were extracted

and the 16S rRNA was amplified by PCR followed by sequencing. Results The obtained specific fragment had a 98% resemblance with 16S rRNA of nanobacteria: Score=2 480 bits (1 290)

Expect=0.0; Identities=1 387/1 409 (98%)

Gaps=4/1 409 (0%); Strand=Plus/Plus. Conclusion Nanobacteria is proved existing in the stones of prostatic calculus patients by PCR and sequencing.

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