pIRES2-FL-IL-3 transfection promotes proliferation of cord blood stem cells

张勇; 张林生; 郭朝华; 张红宾; 董世武

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pIRES2-FL-IL-3 transfection promotes proliferation of cord blood stem cells

更新时间：2026-01-09

- pIRES2-FL-IL-3 transfection promotes proliferation of cord blood stem cells
- Journal of Army Medical University Vol. 29, Issue 24, Pages: 2330-2332(2007)
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  CLC： R329.24;R392.11
- Published：2007
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张勇,张林生,郭朝华,张红宾,董世武.pIRES2-FL-IL-3转染协同促进脐血干细胞增殖的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(24):2330-2332.

　ZHANG Yong,ZHANG Lin-sheng,GUO Chao-hua,ZHANG Hong-bin,DOND Shi-wu.pIRES2-FL-IL-3 transfection promotes proliferation of cord blood stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):2330-2332.
张勇,张林生,郭朝华,张红宾,董世武.pIRES2-FL-IL-3转染协同促进脐血干细胞增殖的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(24):2330-2332. DOI：

　ZHANG Yong,ZHANG Lin-sheng,GUO Chao-hua,ZHANG Hong-bin,DOND Shi-wu.pIRES2-FL-IL-3 transfection promotes proliferation of cord blood stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(24):2330-2332. DOI：

摘要

目的观察真核共表达质粒载体pIRES2-FL-IL-3转染对脐血干细胞增殖的影响及目的蛋白IL-3和FL（Flt3 ligand）的表达变化。方法分为4组：空白对照组（对照组）、pIRES2-IL-3组（IL-3组）、pIRES2-FL组（FL组）和pIRES2-FL-IL-3组（共表达组）。通过台盼蓝染色及MTT法检测pIRES2-FL-IL-3转染（共表达组）对脐血干细胞细胞存活率及增殖的影响，RT-PCR技术、Western blot技术检测脐血干细胞FL和IL-3的mRNA水平和蛋白表达。结果共表达组细胞存活率无显著变化（P0.05），MTT法检测细胞增殖显著高于对照组，共表达组IL-3及FL mRNA水平和蛋白均显著高于对照组，并且高于IL-3组和FL组。真核共表达质粒载体pIRES2-FL-IL-3转染脐血干细胞后细胞存活率无显著变化（P0.05）， 结论脐血干细胞增殖显著增强，目的蛋白能得到有效表达。

Abstract

Objective To observe the proliferation of cord blood stem cells and detect their mRNA and protein levels of FL and IL3 after transfection of plasmid vector pIRES2-FL-IL-3. Methods The cell survival rate and proliferation of the cord blood stem cells were observed by dye staining and MTT methods after transfected by plasmid vector pIRES2-IL-3

pIRES2-FL or pIRES2-FL-IL-3. The mRNA and protein levels of FL and IL-3 were detected by RT-PCR and Western blot. Results No statistical difference on cell survival rate of cord blood stem cells was observed between those transfected by plasmid vector pIRES2-FL-IL-3 and their corresponding controls. The cell proliferation of cord blood stem cells transfected by pIRES2-FL-IL-3 was higher than those transfected by pIRES2-IL-3 or pIRES2-FL. The mRNA and protein levels of FL and IL-3 in the cord blood stem cells transfected by pIRES2-FL-IL-3 were higher than those in the normal cord blood stem cells

and those transfected pIRES2-IL-3 group or pIRES2-FL respectively. Conclusion The transfection of pIRES2-FL-IL-3 to the cord blood stem cells does not influence the cell survival rate but accelerates their proliferation. FL and IL-3 co-expression displays synergic effect on the proliferation of cord blood stem cells.

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