Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines

郭晓; 潘崚

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Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines

更新时间：2026-01-09

- Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines
- Journal of Army Medical University Vol. 30, Issue 5, Pages: 393-395(2008)
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  CLC： R329.28;R73-361;R733.7
- Published：2008
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郭晓,潘崚.ERK阻滞剂U0126对白血病细胞K562细胞周期的作用及机制[J].陆军军医大学学报(原第三军医大学学报),2008,30(05):393-395.

　GUO Xiao,PAN Ling.Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(05):393-395.
郭晓,潘崚.ERK阻滞剂U0126对白血病细胞K562细胞周期的作用及机制[J].陆军军医大学学报(原第三军医大学学报),2008,30(05):393-395. DOI：

　GUO Xiao,PAN Ling.Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(05):393-395. DOI：

摘要

目的研究细胞外信号调节激酶(extracellular signalregulated kinase

ERK)阻滞剂U0126对K562细胞周期的作用及机制。方法以逆转录-聚合酶链反应（reverse transcription-polymerase chain reaction，RT-PCR ）和Western blot检测U0126处理K562细胞后ERK、Cyclin D2、Cyclin E、P27 mRNA和蛋白的表达;以流式细胞术（flow cytometry， FCM）检测细胞周期的变化。结果处理后K562细胞ERK、Cyclin D2、Cyclin E mRNA和蛋白表达降低；P27 mRNA和蛋白表达增高。G

期细胞增多，S期细胞减少，与用药前相比差异均有统计学意义（P0.05）。结论 U0126可能通过ERK途径，影响细胞周期调控蛋白，最终抑制了K562细胞的增殖。

Abstract

Objective To study the mechanism of extracellular signalregulated kinase (ERK) pathway inhibitor U0126 on cell cycle of K562 cell lines. Methods The mRNA and protein expressions of ERK

Cyclin D2

Cyclin E in and P27 K562 cell lines after 10 μmol/L U0126 treatment for 12

24 and 72 h were detected by RT PCR and Western blot

respectively. The cell cycle was determined by flow cytometry. Results The mRNA and protein expressions of ERK

Cyclin D2 and Cyclin E in K562 cell lines after U0126 treatment were decreased

while those of P27 were increased. The percentage of cells in G

phase was increased and that i

n S phase was decreased. All data mentioned above showed significant difference between the K562 cell lines before and after U0126 treatment（P0.05）. Conclusion U0126 possibly affects cell cycle regulating proteins by ERK pathway and eventually inhibits the proliferation of K562 cells.

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