Changes of phenotype and function of human CD4<sup>+</sup>CD25<sup>-</sup> T cells induced after Foxp3 transfection

吴奎; 毕玉田; 王耀丽; 王长征

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Changes of phenotype and function of human CD4⁺CD25^- T cells induced after Foxp3 transfection

更新时间：2026-01-09

- Changes of phenotype and function of human CD4⁺CD25^- T cells induced after Foxp3 transfection
- Journal of Army Medical University Vol. 30, Issue 3, Pages: 186-188(2008)
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  CLC： R392-33;R392.12;{R392.13}
- Published：2008
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吴奎,毕玉田,王耀丽,王长征.Foxp3基因转染对人CD4+ CD25-T细胞表型和功能的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):186-188.

　WU Kui,BI Yu-tian,WANG Yao-li,WANG Chang-zheng.Changes of phenotype and function of human CD4+CD25- T cells induced after Foxp3 transfection[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):186-188.
吴奎,毕玉田,王耀丽,王长征.Foxp3基因转染对人CD4+ CD25-T细胞表型和功能的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):186-188. DOI：

　WU Kui,BI Yu-tian,WANG Yao-li,WANG Chang-zheng.Changes of phenotype and function of human CD4+CD25- T cells induced after Foxp3 transfection[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):186-188. DOI：

摘要

目的探讨体外Foxp3基因表达对CD4

CD25

T细胞功能的影响。方法将编码人Foxp3基因全长的逆转录病毒表达载体，转染人外周血CD4

CD25

T细胞。以流式细胞仪方法检测转染后CD4

CD25

T细胞表面CD25、CD127分子及CTLA4分子的表达变化，以流式细胞仪及RT-PCR检测Foxp3基因在转染细胞中的表达。体外刺激、观察转染后的Foxp3细胞的增殖反应，以及重组细胞对CD4

CD25

T细胞增殖反应和细胞因子分泌的作用。结果转染后Foxp3基因在CD4

CD25

T细胞中呈高水平表达。转染细胞表面CD25、CTLA4分子均呈高水平表达，而CD127分子表达水平下降，且IL-2并不能诱导其增殖。转染细胞与CD4

CD25

T细胞共同培养可有效抑制CD4

CD25

T细胞的增殖反应及细胞因子IL-4、IL-5和IFN-γ的分泌。结论 Foxp3基因转染可有效诱导CD25及CTLA4分子表达，并使转染细胞具有调节性T细胞的功能。

Abstract

Objective To explore the effects of Foxp3 gene transfection on the phenotype and function of CD4

CD25

T cells. Methods The pMSCV-Foxp3 retroviral vector encoding Foxp3 gene was transfected into PT67 packaging cell line. Virus-containing supernatant was applied to differentiated CD4

CD25

T cells

then the cells were sorted with flow cytometry. The expressions of C

D25

CD127

CTLA4 and proliferation of transfected CD4

CD25

T cells were examined. The effect of transfected CD4

CD25

T cells on the proliferation and cytokine production of CD4

CD25

T cells were examined. Results Foxp3 gene transfected CD4

CD25

T cells could express Foxp3. Transfection of Foxp3 gene upregulated the expressions of CD25 and CTLA-4

but down-regulated CD127 expression. After transfection

the proliferation of CD4

CD25

T cells was eliminated. IL-2 could not induce the proliferation of transfected T cells. Transfected T cells inhibited the proliferation of CD4

CD25

T cells after coculture. Conclusion CD4

CD25

T cells acquired a regulatory phenotype and function after transfected with Foxp3 gene

indicating a key role of Foxp3 in the generation of CD4

CD25

regulatory T cells.

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Changes of phenotype and function of human CD4+CD25- T cells induced after Foxp3 transfection

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Abstract

关键词

Keywords

references

Changes of phenotype and function of human CD4⁺CD25^- T cells induced after Foxp3 transfection