Amelioration of Adriamycin nephropathy by transplanting endothelial progenitor cells transfected with Ad-VEGF165 gene

赵洪雯; 余荣杰; 彭侃夫; 刘宏; 吴雄飞; 贺伟峰; 张晓蓉

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Amelioration of Adriamycin nephropathy by transplanting endothelial progenitor cells transfected with Ad-VEGF165 gene

更新时间：2026-01-09

- Amelioration of Adriamycin nephropathy by transplanting endothelial progenitor cells transfected with Ad-VEGF165 gene
- Journal of Army Medical University Vol. 30, Issue 2, Pages: 93-97(2008)
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  CLC： R329;R394.2;R692.02
- Published：2008
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赵洪雯,余荣杰,彭侃夫,刘宏,吴雄飞,贺伟峰,张晓蓉.VEGF165基因转染EPCs移植对大鼠阿霉素肾病组织学的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):93-97.

　ZHAO Hong-wen,YU Rong-jie,PENG Kan-fu,LIU Hong,WU Xiong-fei,HE Wei-feng,ZHANG Xiao-rong.Amelioration of Adriamycin nephropathy by transplanting endothelial progenitor cells transfected with Ad-VEGF165 gene[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):93-97.
赵洪雯,余荣杰,彭侃夫,刘宏,吴雄飞,贺伟峰,张晓蓉.VEGF165基因转染EPCs移植对大鼠阿霉素肾病组织学的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):93-97. DOI：

　ZHAO Hong-wen,YU Rong-jie,PENG Kan-fu,LIU Hong,WU Xiong-fei,HE Wei-feng,ZHANG Xiao-rong.Amelioration of Adriamycin nephropathy by transplanting endothelial progenitor cells transfected with Ad-VEGF165 gene[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):93-97. DOI：

摘要

目的构建含血管内皮生长因子165基因的重组腺病毒(Ad-VEGF

165

)，转染血管内皮祖细胞(EPCs)后，观察其对阿霉素肾病大鼠肾脏组织学的影响。方法用细菌内同源重组法构建含VEGF

165

基因的重组腺病毒Ad-VEGF

165

，通过PCR和Western blot鉴定所构建的腺病毒。贴壁法体外培养获得大鼠骨髓来源的EPCs，Ad-VEGF

165

体外转染EPCs，检测转染后目的基因的蛋白表达。大鼠阿霉素肾病模型形成过程中，尾静脉注射转染Ad-VEGF

165

的EPCs，在第16周时观察肾脏组织学变化。结果 PCR和Western blot证实构建的重组腺病毒含有目的基因，滴度为1.4×10

PFU/ml；培养第6天的EPCs用于Ad-VEGF

165

转染，转染后24 h，培养上清中VEGF蛋白表达较对照组和转染前增加。转染了VEGF

165

基因的EPCs移植后第12周（切肾术后16周），转染组的肾小球硬化和肾小管间质纤维化的程度明显较肾病组轻。结论转染了VEGF

165

基因的EPCs可以减轻阿霉素肾病的肾小球和肾小管的损伤程度，为VEGF在慢性肾脏病治疗提供了实验基础。

Abstract

Objective To construct the recombinant adenovirus of vascular endothelial growth factor 165 (Ad-VEGF

165

) and observe the effects of endothelial progenitor cells (EPCs) transfected with Ad-VEGF

165

gene on ameliorating renal damages induced with Adriamycin. Methods Ad-VEGF

165

was identified with polymerase chain reaction (PCR) and Western blot. EPCs derived from rat bone marrow were obtained b

y adherent culture for 6 days and transfected with Ad-VEGF

165

then injected into tail vein at the early stage of Adriamycin induced renal disease. Results PCR and Western blot indicated that the recombinant adenovirus contained the inserted VEGF

165

. The titer of the purified Ad-VEGF

165

was 1.4×10

pfu/ml. After transfection

the expression of VEGF protein was significantly higher than that of control and untransfected EPCs. The pathological changes were less in VEGF

165

transfecting group than that in untransfecting group at the 12

week

including glomerular sclerosis and tubulointerstitial fibrosis induced by Adriamycin after transplantation. Conclusion The Ad-VEGF

165

effectively mediates the target gene expression in cultured EPCs and ameliorates renal damages. But the adverse effect of VEGF to renal function needs further study.

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