Effects of a new recombinant fusion protein rTMP-GH on proliferation of megakaryocytes in vitro

许杨; 王军平; 赵景宏; 粟永萍; 陈芳; 申明强; 陈默; 王崧; 开丽

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Effects of a new recombinant fusion protein rTMP-GH on proliferation of megakaryocytes in vitro

更新时间：2026-01-09

- Effects of a new recombinant fusion protein rTMP-GH on proliferation of megakaryocytes in vitro
- Journal of Army Medical University Vol. 30, Issue 1, Pages: 1-4(2008)
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  CLC： R331.143;R341;R394.2
- Published：2008
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许杨,王军平,赵景宏,粟永萍,陈芳,申明强,陈默,王崧,开丽.重组融合蛋白rTMP-GH对体外培养巨核细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(01):1-4.

　XU Yang,WANG Jun-ping,ZHAO Jing-hong,SU Yong-ping,CHEN Fang,SHEN Ming-qiang,CHEN Mo,WANG Song,KAI Li.Effects of a new recombinant fusion protein rTMP-GH on proliferation of megakaryocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):1-4.
许杨,王军平,赵景宏,粟永萍,陈芳,申明强,陈默,王崧,开丽.重组融合蛋白rTMP-GH对体外培养巨核细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(01):1-4. DOI：

　XU Yang,WANG Jun-ping,ZHAO Jing-hong,SU Yong-ping,CHEN Fang,SHEN Ming-qiang,CHEN Mo,WANG Song,KAI Li.Effects of a new recombinant fusion protein rTMP-GH on proliferation of megakaryocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(01):1-4. DOI：

摘要

目的探讨TPO模拟肽（thrombopoietin mimetic peptide

TMP）与人生长激素(growth hormone

GH)的重组融合蛋白rTMP-GH对体外培养巨核系细胞增殖的影响，以及对血小板生成有显著调控作用的球蛋白转录调节因子(globin transcription factor-1

GATA-1)表达的调控作用。方法体外培养Dami细胞，100 ng/ml的重组融合蛋白rTMP-GH作用7 d后，观察巨核细胞集落形成能力，分析rTMPGH对Dami细胞增殖的影响。同时，采用Western blot和RT-PCR方法分别检测Dami细胞中GATA-1 的蛋白水平和mRNA表达变化，探讨rTMP-GH对巨核细胞生成血小板的调节作用。结果 rTMP-GH处理后Dami细胞的集落形成能力显著增强，转录因子GATA-1的mRNA和蛋白表达水平显著上调，其调节作用明显强于空白对照组和GH组。结论 rTMP-GH能够刺激巨核细胞增殖分化和上调GATA-1的表达，具有促进血小板生成的作用。

Abstract

Objective To investigate the effects of rTMP-GH

a recombinant fusion protein of thrombopoietin mimetic peptide (TMP) and human growth hormone (GH)

on the proliferation and thrombocytopoiesis of cultured megakaryocytes. Methods After being treated with 100 ng/ml of rTMP-GH for 7 d

Dami cells

a kind of megakaryocyte cell line

were analyzed by observing the numbers of colony forming unit. Meanwhile

Western blotting and RT-PCR were applied to detect the expression changes of globin transcription factor-1 (GATA-1)

which is a main regulator of thrombocytopoiesis in megakaryocytes. Results The numbers of colony forming unit were markedly increased in cultured Dami cells incubated with rTMP-GH. Compared with normal control and GH treatment groups

both the mRNA and protein levels of GATA-1 were up-regulated significantly in Dami cells treated with rTMP-GH. Conclusion rTMP-GH has a strong ability to promote the proliferation and thrombocytopoiesis of megakaryocytes.

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