Effect of mimic CO<sub>2</sub> pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells

石彦; 郝迎学; 雷晓; 余佩武; 钱锋; 唐波; 赵永亮; 饶云

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Effect of mimic CO₂ pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells

更新时间：2026-01-09

- Effect of mimic CO₂ pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells
- Journal of Army Medical University Vol. 29, Issue 23, Pages: 2274-2276(2007)
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  CLC： R656;R730.23;R735.2
- Published：2007
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石彦,郝迎学,雷晓,余佩武,钱锋,唐波,赵永亮,饶云.模拟CO2气腹对人胃癌MKN-45细胞增殖和细胞周期的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2274-2276.

　SHI Yan,HAO Ying-xue,LEI Xiao,YU Pei-wu,QIAN Feng,TANG Bo,ZHAO Yong-liang,RAO Yun.Effect of mimic CO2 pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2274-2276.
石彦,郝迎学,雷晓,余佩武,钱锋,唐波,赵永亮,饶云.模拟CO2气腹对人胃癌MKN-45细胞增殖和细胞周期的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2274-2276. DOI：

　SHI Yan,HAO Ying-xue,LEI Xiao,YU Pei-wu,QIAN Feng,TANG Bo,ZHAO Yong-liang,RAO Yun.Effect of mimic CO2 pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2274-2276. DOI：

摘要

目的通过建立体外模拟CO

气腹环境，研究CO

气腹对人胃癌MKN-45细胞增殖和细胞周期的影响。方法建立体外模拟CO

的气腹环境，在全自动气腹机作用下维持密闭培养箱内压力为12 mmHg，作用时间4 h。处理后用MTT法检测细胞增殖情况；通过流式细胞仪观察细胞周期变化。结果 MTT法检测细胞增殖结果显示，气腹组人胃癌MKN-45细胞的增殖速度与对照组比较无显著性差异（P＞0.05）；流式细胞仪检测人胃癌MKN-45细胞周期，CO

气腹组增殖指数与对照组比较无显著性差异（P＞0.05）。结论体外模拟持续12 mmHg CO

气腹环境，连续对人胃癌MNK-45细胞作用4 h，不会促进胃癌细胞的增殖和生长。

Abstract

Objective To study the effects of mimic carbon dioxide pneumoperitoneum on cell proliferation of human gastric cancer cells. Methods The gastric cancer cells MKN-45 were exposed to CO

environment for 4 h under the pressure of 12 mmHg and those cultured in normal experiment condition as control. The cell proliferation of MKN-45 cells were examined by MTT method on day 1-7 after exposure and their cell cycle by flow cytometry 24 h after exposure. Results The cell proliferation and cell proliferative index (PI) showed no statistical difference between CO

pneumoperitoneum group and control group (P0.05). Conclusion The ce

ll proliferation and cell cycle of human gastric cancer MKN-45 are not significantly affected after exposure to CO

pneumoperitoneum.

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Effect of mimic CO2 pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells

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摘要

Abstract

关键词

Keywords

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Effect of mimic CO₂ pneumoperitoneum on cell proliferation and cell cycle of human gastric cancer MKN-45 cells