Mutation analysis on PB1 gene of human avian influenza H<sub>5</sub>N<sub>1</sub> virus

黄平; 俞守义; 柯昌文; 邹丽容; 方苓; 李晖; 陈秋霞

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Mutation analysis on PB1 gene of human avian influenza H₅N₁ virus

更新时间：2026-01-09

- Mutation analysis on PB1 gene of human avian influenza H₅N₁ virus
- Journal of Army Medical University Vol. 29, Issue 22, Pages: 2168-2170(2007)
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  CLC： Q754;R373.13
- Published：2007
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黄平,俞守义,柯昌文,邹丽容,方苓,李晖,陈秋霞.人禽流感H5N1毒株PB1基因突变分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2168-2170.

　HUANG Ping,YU Shou-yi,KE Chang-wen,ZOU Li-rong,FANG Ling,LI Hui,CHEN Qiu-xia.Mutation analysis on PB1 gene of human avian influenza H5N1 virus[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2168-2170.
黄平,俞守义,柯昌文,邹丽容,方苓,李晖,陈秋霞.人禽流感H5N1毒株PB1基因突变分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2168-2170. DOI：

　HUANG Ping,YU Shou-yi,KE Chang-wen,ZOU Li-rong,FANG Ling,LI Hui,CHEN Qiu-xia.Mutation analysis on PB1 gene of human avian influenza H5N1 virus[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2168-2170. DOI：

摘要

目的通过对人禽流感H

毒株PB1基因序列的变异分析，揭示毒株PB1基因的特征与进化。方法检测广东地区人禽流感H

毒株PB1基因核苷酸序列，同时检索全球人禽流感H

毒株PB1基因序列，采用DNA Star Lasergene软件对检索的人禽流感H

毒株PB1基因核苷酸序列进行比对和分析，并结合流行病学资料对变异毒株进行进化速度分析。结果将50株毒株PB1基因核苷酸序列按同源性分成两组：1997-1998年毒株为第1组（G1），2003-2006年毒株为第2组（G2）。PB1基因74个氨基酸发生位点置换，占9.78％(74/757)。PB1基因Ks值为26.1×10

-6

～39.8×10

-6

Nt/d，Ka值为3.91×10

-6

～5.59×10

-6

Nt/d，检验结果显示，基因进化受到负选择性压力。2003-2006年毒株（G2）丢失G

757

，引起氨基酸结构改变；同时糖基化位点也较1997-1998年毒株减少了1个糖基化位点NES

382-384

。结论目前PB1基因进化分成两组，自发突变作用影响PB1基因进化；PB1基因与PB2基因的进化途径在时间特征和区域特征方面类似。2003-2006年毒株PB1基因氨基酸结构和糖基化位点均有改变。

Abstract

Objective To reveal the characteristics and revolutions of PB1 gene of human avian influenza H

subtype by analyzing its mutation. Methods The PB1 gene of H

viruses isolated in Guangdong was sequenced

and the global PB1 genes of the viruses were obtained from internet；They were analyzed by DNAStar Lasergene by me

ans of studying the revolutionary speeds with the epidemiological data. Results PB1 genes of 50 strains during 1997 to 2006 were homologouslly classified into 2 groups

the strains from Hong Kong during 1997 to 1998 (G1) and the strains during 2003 to 2006 (G2).Totally 74 amino acids were substituted in PB1 gene in all strains，with ratio of 9.78%（74/757）. In the synonymous variation，Ks values in PB1 were 26.1×10

-6

to 39.8×10

-6

Nt/d and Ka values in PB1 were 3.91×10

-6

to 5.59×10

-6

Nt/d，where there existed obviously the automatic substitution and obviously negative selective pressure by biological test.G2 strain were found with a deletion of a glycine (757)

and G1 strains had a lose of glycosylation site of NES

382-384

. Conclusion PB1 genes were classified into 2 groups and evolved rapidly by spontaneous substitution，which is similar to PB2 genes in gene evolution between time feature and district feature. Deletions of G

757

and NES

382-384

in strains during 2003 to 2006 are worth further researching about clinical significance in the future.

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Mutation analysis on PB1 gene of human avian influenza H5N1 virus

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Mutation analysis on PB1 gene of human avian influenza H₅N₁ virus