Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs

唐仕明; 何大朋; 王忠; 辜福贤; 王明荣

您当前的位置：

首页 >

文章列表页 >

Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs

更新时间：2026-01-09

- Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs
- Journal of Army Medical University Vol. 29, Issue 23, Pages: 2264-2266(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.62;R329.28
- Published：2007
- 稿件说明：
移动端阅览
唐仕明,何大朋,王忠,辜福贤,王明荣.抑制Kit信号通路对豚鼠膀胱间质细胞增殖及自主节律性运动的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2264-2266.

　TANG Shi-ming,HE Da-peng,WANG Zhong,GU Fu-xian,WANG Ming-rong.Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2264-2266.
唐仕明,何大朋,王忠,辜福贤,王明荣.抑制Kit信号通路对豚鼠膀胱间质细胞增殖及自主节律性运动的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2264-2266. DOI：

　TANG Shi-ming,HE Da-peng,WANG Zhong,GU Fu-xian,WANG Ming-rong.Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2264-2266. DOI：

摘要

目的探讨抑制Kit表达对豚鼠膀胱间质细胞(interstitial cells

ICs)增殖及自主节律性运动的影响。方法成年豚鼠分别予伊马替尼灌胃20 d和40 d，免疫荧光、Western blot和体外灌流观察间质细胞形态和功能的变化。结果正常豚鼠膀胱壁内可见较多的间质细胞，用药20 d和40 d后，细胞数量逐渐减少，Kit蛋白的表达逐渐降低，同时，膀胱的自主节律性运动受到抑制。结论膀胱内含间质细胞，这些细胞可能是膀胱运动的起搏细胞，其细胞表面表达的Kit蛋白对间质细胞的存活和功能维持都具有重要的作用。

Abstract

Objective To investigate the role of Kit signal pathway in the proliferation and spontaneous rhythmic motility of interstitial cells (ICs) in the bladder of guinea pigs. Methods Fourteen Guinea pigs aged 45-60 days were administered with Imatinib intragastrically for 20 and 40 days. Three were fed on normal saline as control. The morph and number of ICs were observed by immunofluorescence

Kit protein expression was detected by Western blot and spontaneous rhythmic motility of bladder were investigated in in vitro organ bath. Results Quite a few ICs were observed in the bladder of controls. After administration of Imatinib

the amount of ICs and the expression of Kit protein decreased gradually

and the spontaneous rhythmic contraction weakened. Conclusion ICs might be the pacemaker of bladder in guinea pigs. Kit protein might play an important role in the survival and functional maintenance of ICs.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Toosendanin inhibits proliferation and promotes apoptosis of gastrointestinal stromal tumor-T1 cells by down-regulating c-kit expression

Anti-ICAM targeted liposomes loaded with imatinib and liquid fluorocarbon treat ALI/ARDS mouse model induced by LPS

Knockdown of scinderin inhibits proliferation in gastrointestinal stromal tumor cells

Kit signaling regulates phenotype of interstitial cells of Cajal in adult mice

Related Author

Xi ZHANG

Yunfang ZHANG

Zheng WANG

Xiaoqi WANG

Yuxuan DU

Weihao WANG

Tao HONG

Ruihao HUANG

Related Institution

State Key Laboratory of Trauma, Burns and Combined Injury, Medical Center of Hematology, PLA Center for Hematology, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Clinical Laboratory, the Second Affiliated Hospital of Zunyi Medical University

School of Medicine, Southwest Jiaotong University

Section of Gastrointestinal Diseases, Center for General Surgery, General Hospital of Western Theater Command

School of Clinical Medicine, Southwest Medical University

⁰