Rapid detection of TSH and T<sub>4</sub> by microfluidic chip-based immunoassays

杨帆; 黄辉; 蒲晓允

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Rapid detection of TSH and T₄ by microfluidic chip-based immunoassays

更新时间：2026-01-09

- Rapid detection of TSH and T₄ by microfluidic chip-based immunoassays
- Journal of Army Medical University Vol. 29, Issue 23, Pages: 2254-2256(2007)
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  CLC： R319;R446.6
- Published：2007
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杨帆,黄辉,蒲晓允.微流控免疫芯片快速检测法的建立及其检测TSH和T4的效果[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2254-2256.

　YANG Fan,HUANG Hui,PU Xiao-yun.Rapid detection of TSH and T4 by microfluidic chip-based immunoassays[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2254-2256.
杨帆,黄辉,蒲晓允.微流控免疫芯片快速检测法的建立及其检测TSH和T4的效果[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2254-2256. DOI：

　YANG Fan,HUANG Hui,PU Xiao-yun.Rapid detection of TSH and T4 by microfluidic chip-based immunoassays[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2254-2256. DOI：

摘要

目的在已制备的微流控芯片系统上建立基于顺磁微球的免疫检测方法，并将此方法用于检测促甲状腺激素（TSH）和甲状腺素（T

）。方法采用数字激光刻蚀技术制备微流控芯片，以luminol-H

为发光体系，用双抗夹心法测定TSH，用免疫竞争法测定T

。结果该检测系统可以在25 min内完成检测，所需的标本和抗体试剂量为10 μl，TSH检测范围为0～50 μIU/ml，批内CV平均为4.9％，批间CV平均为4.9％，平均回收率为93.74％；T

检测范围为9.375～300 ng/ml，批内CV平均为4.9％，批间CV平均为4.7％，平均回收率为91.27％。结论本方法检测TSH和T

具有操作简便、节约时间、节省试剂、稳定性好、准确度高的优点。

Abstract

Objective To establish an immunoassay based on microfluidic chip for detection of TSH and total T

. Methods The direct-write laser micromachining technology was used to fabricate microfluidic chip

luminol-H

as luminator. This method and Roche E2010 electrochemiluminescence immunoassay were used to examine TSH by double-antibody sandwich method and total T

by competitive principle in 50 serum samples collected at our outpatient clinic. Results The detection was completed within 25 min. Ten microliter sample and reagent was required. The detection range of TSH was 0-50 μIU/ml and its average intraassay and interassay

precision was 4.7% and 4.6% respectively and its average recovery rate was 95.3%. The detection range of T

was 9.375-300 ng/ml

and its average intraassay and interassay precision was 4.9% and 4.7% respectively and its average recovery rate was 91.27%. Conclusion Rapid detection of TSH and T

by microfluidic chip-based immunoassays is convenient

stable and accurate and of less reagent and time consuming.

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Rapid detection of TSH and T4 by microfluidic chip-based immunoassays

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Rapid detection of TSH and T₄ by microfluidic chip-based immunoassays