Cytotoxicity of NK cells inhibited by CD4<sup>+</sup>T cells stimulated by ConA

王庆红; 王晓娟; 甘成军; 彭旭; 罗高兴; 吴军

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Cytotoxicity of NK cells inhibited by CD4⁺T cells stimulated by ConA

更新时间：2026-01-09

- Cytotoxicity of NK cells inhibited by CD4⁺T cells stimulated by ConA
- Journal of Army Medical University Vol. 29, Issue 22, Pages: 2145-2147(2007)
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  CLC： R392-33;R392.12
- Published：2007
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王庆红,王晓娟,甘成军,彭旭,罗高兴,吴军.TGF-β1在ConA激活的CD4+T细胞抑制NK细胞杀伤毒性中的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2145-2147.

　WANG Qing-hong,WANG Xiao-juan,GAN Cheng-jun,PENG Xu,LUO Gao-xing,WU Jun.Cytotoxicity of NK cells inhibited by CD4+T cells stimulated by ConA[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2145-2147.
王庆红,王晓娟,甘成军,彭旭,罗高兴,吴军.TGF-β1在ConA激活的CD4+T细胞抑制NK细胞杀伤毒性中的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2145-2147. DOI：

　WANG Qing-hong,WANG Xiao-juan,GAN Cheng-jun,PENG Xu,LUO Gao-xing,WU Jun.Cytotoxicity of NK cells inhibited by CD4+T cells stimulated by ConA[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2145-2147. DOI：

摘要

目的初步探讨ConA激活的CD4

T细胞对NK细胞杀伤毒性的影响及TGF-β

分子在其中的作用。方法磁珠分选纯化BALB/c小鼠脾脏CD4

T细胞与NK细胞，体外用ConA激活CD4

T细胞，将激活的CD4

T细胞与NK细胞共培养，并加入TGF-β

抗体，共培养24、48、72 h和96 h后，用

Cr标记的YAC-1检测NK细胞的杀伤毒性。结果 ConA 激活的CD4

T细胞能够在所测的4个时相点显著抑制NK细胞的杀伤毒性，抑制率分别为25.00%、31.60%、34.70%和46.50%；在此共培养体系中加入TGF-β

抗体后，抑制率分别增加到63.80%、59.43%、60.70%和90.90%。结论 ConA 激活的CD4

T细胞能够显著抑制NK细胞的杀伤毒性，并且这种抑制作用随着TGF-β

抗体的加入而增强，表明TGF-β

分子影响了ConA激活的CD4

T细胞对NK细胞杀伤毒性的抑制。

Abstract

Objective To explore the effect of CD4

T cells stimulated by ConA on the cytotoxic activity of NK cells. Methods CD4

T cells and NK cells were purified from spleen of BALB/c mice spleen by magnetic cell sorting. CD4

T cells stimulated by ConA and NK cells were co-cultured with or without anti-TGF-β

antibody in the co-culture system. The cytotoxicity of NK cells was assayed by YAC-1 labeled by

Cr in 24

72 and 96 h. Resul

ts The cytotoxicity of NK cells were inhibited significantly by CD4

T cells activated by ConA at 4 time points and the inhibitory rate respectively was 25%

31.6%

34.7% and 46.5%. After anti-TGF-β

antibody was added into the co-culture system

the inhibitory rate reached to 63.8%

59.43%

607% and 90.9%. Conclusion In vitro

CD4

T cells stimulated by ConA notably inhibits the cytotoxicity of NK cells

and TGF-β

pathway may be involved in this action.

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Cytotoxicity of NK cells inhibited by CD4+T cells stimulated by ConA

DOI：

摘要

Abstract

关键词

Keywords

references

Cytotoxicity of NK cells inhibited by CD4⁺T cells stimulated by ConA