Hematopoiesis reconstruction in mice of hematopoietic aplasia by transplanting hematopoietic stem/progenitor cells mobilized by Angelica polysaccharides

胡晶; 冯敏; 杨慧; 吴宏

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Hematopoiesis reconstruction in mice of hematopoietic aplasia by transplanting hematopoietic stem/progenitor cells mobilized by Angelica polysaccharides

更新时间：2026-01-09

- Hematopoiesis reconstruction in mice of hematopoietic aplasia by transplanting hematopoietic stem/progenitor cells mobilized by Angelica polysaccharides
- Journal of Army Medical University Vol. 29, Issue 23, Pages: 2236-2239(2007)
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  CLC： R285.5;R331.2;R551.05
- Published：2007
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胡晶,冯敏,杨慧,吴宏.当归多糖动员的造血干/祖细胞移植重建小鼠造血功能的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2236-2239.

　HU Jing,FENG Min,YANG Hui,WU Hong.Hematopoiesis reconstruction in mice of hematopoietic aplasia by transplanting hematopoietic stem/progenitor cells mobilized by Angelica polysaccharides[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2236-2239.
胡晶,冯敏,杨慧,吴宏.当归多糖动员的造血干/祖细胞移植重建小鼠造血功能的研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(23):2236-2239. DOI：

　HU Jing,FENG Min,YANG Hui,WU Hong.Hematopoiesis reconstruction in mice of hematopoietic aplasia by transplanting hematopoietic stem/progenitor cells mobilized by Angelica polysaccharides[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(23):2236-2239. DOI：

摘要

目的探讨当归多糖（Angelica polysaccharides

APS）动员的造血干/祖细胞（hematopoietic stem/progenitor cell， HSPC）对造血衰竭小鼠造血功能重建的作用。方法将APS动员的雄性BALB/c小鼠的外周血单个核细胞（peripheral blood mononuclear cells

PBMC）静脉输注给受到8.5 Gy

Co γ射线照射的雌性同系受体小鼠；观察受体小鼠外周血白细胞（WBC）、血红蛋白（HGB）、血小板（PLT）的变化及30 d存活情况；采用PCR方法鉴定其造血重建的来源。结果 APS组受体小鼠WBC、PLT、HGB、30 d存活数均明显高于对照组和NS组（P0.05）；对APS组存活小鼠进行Y染色体PCR分析，证实雌性受体小鼠重建的造血细胞来自雄性供体。结论 APS动员的小鼠外周血HSPC移植后能够重建造血衰竭小鼠长期造血。

Abstract

Objective To study hematopoiesis reconstruction in mice of hematopoietic aplasia by transplanting hematopoietic stem/progenitor cells mobilized by Angelica polysaccharides (APS). Methods The female BALB/c mice irradiated with 8.5 Gy

Co γ ray were transplanted with peripheral blood mononuclear cells (PBMCs) from male BALB/c mice mobilized by APS. After transplantation

the changes of WBC

HGB and platelet in peripheral blood were observed. Thirty-day survival rate was calculated. PCR was used to verify the cell source of hematopoiesis reconstruction. Res

ults The number of WBC

platelet and HGB

and 30-day survival rate of APS group were markedly higher than that of negative control group and normal saline group (P0.05). PCR results showed Y chromosome sequence existed in the survived mice of APS group

indicating that the repopulated hematopoietic cells came from the male donor. Conclusion The hematopoietic stem/progenitor cells mobilized by APS can reconstruct long-term hematopoiesis in mice of hematopoietic aplasia.

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