Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells

申丽丽; 彭家和; 刘红; 彭彦孟; 甘淋; 鲁立; 张迁; 江渝

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Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells

更新时间：2026-01-09

- Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells
- Journal of Army Medical University Vol. 29, Issue 22, Pages: 2142-2144(2007)
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  CLC： Q591.5;R394;R541.402
- Published：2007
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申丽丽,彭家和,刘红,彭彦孟,甘淋,鲁立,张迁,江渝.法尼酯X受体对脂肪酸合酶表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2142-2144.

　SHEN Li-li,PENG Jia-he,LIU Hong,PENG Yan-meng,GAN Lin,LU Li,ZHANG Qian,JIANG Yu.Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2142-2144.
申丽丽,彭家和,刘红,彭彦孟,甘淋,鲁立,张迁,江渝.法尼酯X受体对脂肪酸合酶表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2142-2144. DOI：

　SHEN Li-li,PENG Jia-he,LIU Hong,PENG Yan-meng,GAN Lin,LU Li,ZHANG Qian,JIANG Yu.Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2142-2144. DOI：

摘要

目的探讨法尼酯X受体(farnesoid X receptor

FXR)对脂肪酸合酶（fatty acid synthetase

FAS）表达的影响。方法用FXR激动剂CDCA作用人肝癌细胞株（HepG2），应用 RT-PCR和Western blotting分别从mRNA水平和蛋白质水平检测FAS的表达情况。结果用不同浓度的CDCA（25、50、75 μmol/L）分别作用于HepG2细胞，6、12、24、48 h后，FAS mRNA和蛋白质水平呈时间和剂量依赖性下调。结论 FXR激动剂可抑制脂肪酸合酶的表达。

Abstract

Objective To investigate the effect of the farnesoid X receptor (FXR) on fatty acid synthetase expression. Methods Human hepatoblastoma HepG2 cells were treated with a FXR’s agonist

chenodeoxycholic acid (CDCA) at 75 μmol/L for 6

24 and 48 h respectively

or at 25

50 and 75 μmol/L for 48 h. Expressions of FAS mRNA and protein were determined by RT-PCR and Western blot analysis respectively. Results RT-PCR and Western blot analysis indicated that the mRNA and protein were expressed in a time- and dose-dependent manner in the HepG2 cells treatmented by CDCA. Conclusion FXR agonist decreases the expression of fatty acid synthetase.

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