Inhibition of HeLa cell proliferation by cyclinE RNA interference

陈济; 潘巍巍; 成海恩; 魏丽丽; 袁成福; 石华; 易发平; 马永平; 宋方洲

您当前的位置：

首页 >

文章列表页 >

Inhibition of HeLa cell proliferation by cyclinE RNA interference

更新时间：2026-01-09

- Inhibition of HeLa cell proliferation by cyclinE RNA interference
- Journal of Army Medical University Vol. 29, Issue 22, Pages: 2135-2138(2007)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394-33;R73-362;R737.33
- Published：2007
- 稿件说明：
移动端阅览
陈济,潘巍巍,成海恩,魏丽丽,袁成福,石华,易发平,马永平,宋方洲.细胞周期素E的干扰RNA对HeLa细胞增殖的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2135-2138.

　CHEN Ji,PAN Wei-wei,CHENG Hai-en,WEI Li-li,YUAN Cheng-fu,SHI Hua,YI Fa-ping,MA Yong-ping,SONG Fang-zhou.Inhibition of HeLa cell proliferation by cyclinE RNA interference[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2135-2138.
陈济,潘巍巍,成海恩,魏丽丽,袁成福,石华,易发平,马永平,宋方洲.细胞周期素E的干扰RNA对HeLa细胞增殖的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2135-2138. DOI：

　CHEN Ji,PAN Wei-wei,CHENG Hai-en,WEI Li-li,YUAN Cheng-fu,SHI Hua,YI Fa-ping,MA Yong-ping,SONG Fang-zhou.Inhibition of HeLa cell proliferation by cyclinE RNA interference[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2135-2138. DOI：

摘要

目的利用RNAi技术下调宫颈癌HeLa细胞中cyclin E mRNA水平，研究对HeLa细胞增殖及周期的影响。方法构建针对cyclin E基因的RNAi真核表达载体，脂质体转染HeLa细胞下调细胞中cyclin E mRNA含量。RT-PCR检测抑制效果；MTT检测细胞生长情况；流式细胞仪分析HeLa细胞周期变化。结果成功构建针对cyclin E基因的RNAi真核表达载体，可以特异下调cyclin E mRNA含量，并抑制细胞恶性增殖。细胞周期分析显示干扰使细胞停留在G

期，S期细胞明显减少。结论 RNAi能特异地下调HeLa 细胞中cyclin E mRNA含量，抑制宫颈癌细胞恶性增殖，提示cyclin E 可能成为宫颈癌基因治疗中的一个新的靶点。

Abstract

Objective To study the effect of RNAi targeting cyclin E on the proliferation and cell cycle of cervical cancer cell line HeLa. Methods The eukaryotic expression vector of cyclin E RNAi was constructed and transfected into HeLa cell. The mRNA level of cyclinE was detected by RT-PCR. Cell proliferation was analyzed by MTT；Cell cycle was analyzed by flow cytometry. Results The vector of cyclinE RNAi was successfully constructed

and specially knocked down the mRNA level of cyclin E gene after transfection. MTT showed the cell proliferation was inhibited. Cells stopped at G

-G

phase and cell number in S phase was sharply decreased after RNAi. Conclusion The mRNA level of cycli

nE gene in HeLa cell can be specially knocked down by RNAi

and the malignant proliferation of cervical cancer cells is inhibited too.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of LncRNA LINC01140 on proliferation and cell cycle in acute myeloid leukemia U937 cells

Nanopatch-delivered HPV18 E7 siRNA inhibits proliferation of skin cells in cervical cancer transgenic mice

Runx3 improves chemosensitivity of hepatocellular carcinoma cells HepG2 to cisplatin

RNA interference targeting EGFR on proliferation of human ovarian carcinoma cell line SKOV₃

Effect of siRNA mediated down-regulation of AnnexinⅡ on human breast cancer cell line MDA-MB-435S

Related Author

Qingsong LUO

Xiaoye HU

Yao ZHU

Ning GAO

熊倬

董晓静

孙培文

张莹

Related Institution

Department of Pharmacognosy, Faculty of Pharmacy & Laboratory Medicine, Army Medical University (Third Military Medical University)

Department of Gynecology and Obstetrics， the First Affiliated Hospital of Chongqing Medical University

Department of Gynecology and Obstetrics， Women and Children’s Hospital Affiliated to Chongqing Medical University

Treatment Center of Radiation Oncology, Chongqing University Cancer Hospital

Gynecological Oncology Center, Chongqing University Cancer Hospital

⁰