Molecular cloning, sequence analysis of mouse integrin α<sub>4</sub> gene and repairing of its mutation

王永明; 胡燕; 黎海芪

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Molecular cloning, sequence analysis of mouse integrin α₄ gene and repairing of its mutation

更新时间：2026-01-09

- Molecular cloning, sequence analysis of mouse integrin α₄ gene and repairing of its mutation
- Journal of Army Medical University Vol. 29, Issue 22, Pages: 2131-2134(2007)
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  CLC： R322.45;R394-33;R394.3
- Published：2007
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王永明,胡燕,黎海芪.小鼠整合素α4基因克隆、序列分析及突变修复[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2131-2134.

　WANG Yong-ming,HU Yan,LI Hai-qi.Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2131-2134.
王永明,胡燕,黎海芪.小鼠整合素α4基因克隆、序列分析及突变修复[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2131-2134. DOI：

　WANG Yong-ming,HU Yan,LI Hai-qi.Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2131-2134. DOI：

摘要

目的克隆小鼠整合素α

基因cDNA，进行分析整合素α

基因序列，对造成氨基酸突变的碱基进行定点突变修复。方法从小鼠小肠peyer’s结获得α

基因cDNA，采用RT-PCR方法，克隆到pMD18-T载体，选择阳性克隆进行酶切鉴定和序列测定，对造成氨基酸突变的碱基进行定点突变修复。结果扩增得到的α

基因cDNA为3 099 bp，编码1 032 个氨基酸，与GenBank中公布的序列比较，有12个碱基发生突变，其中6个碱基造成6个氨基酸发生改变，对发生突变的6个碱基进行定点突变修复。结论获得小鼠整合素α

基因的克隆。

Abstract

Objective To clone and analyze the full-length cDNA of mouse integrin α

and repair the mutation sensible locei that caused the change of amino acids. Methods The cDNA of α

gene was amplified by RT-PCR using the total RNA extracted from mouse small intestine peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed into E. coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of α

cDNA that caused the change of amino acids was repaired. Results The cDNA of mouse α

had a length of 3 099 bp

and encoded a product of 1 032 amino acids. There were 12 bases pairs mutati

on of α4 gene and the 6 base pairs causing the change of amino acids was repaired. Conclusion The cDNA of mouse α

is cloned successfully.

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Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation

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Molecular cloning, sequence analysis of mouse integrin α₄ gene and repairing of its mutation