Differential microRNA expression profile of HepG2 cells induced by HBV integration

韩俊峰; 阮志华; 张蓓; 吴玉章

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Differential microRNA expression profile of HepG2 cells induced by HBV integration

更新时间：2026-01-09

- Differential microRNA expression profile of HepG2 cells induced by HBV integration
- Journal of Army Medical University Vol. 29, Issue 22, Pages: 2128-2130(2007)
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  CLC： R322.47;R394.3;R512.620.2
- Published：2007
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韩俊峰,阮志华,张蓓,吴玉章.HBV整合引起HepG2细胞microRNA表达谱变化分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2128-2130.

　HAN Jun-feng,RUAN Zhi-hua,ZHANG Bei,WU Yu-zhang.Differential microRNA expression profile of HepG2 cells induced by HBV integration[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2128-2130.
韩俊峰,阮志华,张蓓,吴玉章.HBV整合引起HepG2细胞microRNA表达谱变化分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2128-2130. DOI：

　HAN Jun-feng,RUAN Zhi-hua,ZHANG Bei,WU Yu-zhang.Differential microRNA expression profile of HepG2 cells induced by HBV integration[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2128-2130. DOI：

摘要

目的分析HBV整合后引起的肝细胞microRNA表达谱的变化情况。方法利用microRNA芯片，以稳定表达HBV的HepG2.2.15细胞为HBV感染模型，以HepG2细胞为对照，分析二者microRNA表达谱的异同，得到因HBV感染而产生变化的microRNA；并用定量PCR技术来验证结果的可靠性。结果通过microRNA芯片分析和定量PCR验证，HBV感染后可使hsa-miR-30a-5p、hsa-miR-24、hsa-let-7a、hsa-let-7c、hsa-let-7f和hsa-miR-23b 6种microRNA下调，使hsa-miR-194、hsa-miR-200a和hsa-miR-345 3种microRNA上调。结论 HBV整合后，可引起肝脏细胞microRNA表达谱变化。

Abstract

Objective To investigate the differential expressions of microRNA after HBV integration. Methods A human microRNA microarray was used to analyze the microRNA expression profile in HepG2.2.15 cell line

a novel model of HBV infection. HepG2 cells served as a mock control. Then the microRNA with differential expression were proved using real-time PCR. Results HBV infection induced 6 microRNA down-regulated (hsa-miR-30a-5p

hsa-miR-24

hsa-let-7a

hsa-let-7c

hsa-let-7f and hsa-miR-23b) and 3 microRNA up-regulated (hsa-miR-194

hsa-miR-200a and hsa-miR-345). Conclusion HBV integration can induce the changes of microRNA expression profile in hepatocytes.

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