Prediction and peptide binding assay of HLA-A2.1-restricted CTL epitope from heparanase

陈婷; 万瑛; 汤旭东; 房殿春; 余松涛; 熊震; 罗元辉; 杨仕明

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Prediction and peptide binding assay of HLA-A2.1-restricted CTL epitope from heparanase

更新时间：2026-01-09

- Prediction and peptide binding assay of HLA-A2.1-restricted CTL epitope from heparanase
- Journal of Army Medical University Vol. 29, Issue 22, Pages: 2120-2123(2007)
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  CLC： R394-33;R394.3;R730.2
- Published：2007
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陈婷,万瑛,汤旭东,房殿春,余松涛,熊震,罗元辉,杨仕明.人肿瘤转移相关抗原肝素酶HLA-A2.1限制性CTL表位的预测及初步鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2120-2123.

　CHEN Ting,WAN Ying,TANG Xu-dong,FANG Dian-chun,YU Song-tao,XIONG Zhen,LUO Yuan-hui,YANG Shi-ming.Prediction and peptide binding assay of HLA-A2.1-restricted CTL epitope from heparanase[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2120-2123.
陈婷,万瑛,汤旭东,房殿春,余松涛,熊震,罗元辉,杨仕明.人肿瘤转移相关抗原肝素酶HLA-A2.1限制性CTL表位的预测及初步鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2120-2123. DOI：

　CHEN Ting,WAN Ying,TANG Xu-dong,FANG Dian-chun,YU Song-tao,XIONG Zhen,LUO Yuan-hui,YANG Shi-ming.Prediction and peptide binding assay of HLA-A2.1-restricted CTL epitope from heparanase[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2120-2123. DOI：

摘要

目的预测并鉴定肿瘤转移相关抗原肝素酶来源的HLA-A2.1限制性CTL表位。方法采用超基序、量化基序和分子模拟相结合的方法，对肝素酶HLA-A2.1限制性CTL表位进行预测，合成候选表位肽；利用T

细胞的特点，对合成的候选肽与HLA-A2.1分子进行亲和力分析，初步验证预测结果；利用标准

Cr释放试验检测特异性CTLs诱导活性。结果在所筛选的5个候选CTL表位中

Hpa(525-533)、Hpa(277-285)、Hpa(405-413)可在体外有效诱导肝素酶特异性CTLs的产生

对肝素酶阳性且HLA-A2阳性的KATOⅢ细胞具有明显的杀伤效应。结论 Hpa(525-533)、Hpa(277-285)、Hpa(405-413)有可能是肿瘤抗原肝素酶HLA-A2.1的限制性CTL 表位。

Abstract

Objective To predict and identify the HLA-A2.1-restricted cytotoxic T lymphocyte (CTL) epitopes in heparanase (Hpa)

a new tumor associated antigen. Methods Supermotif and quantitative motif methods were used to predict the CTL epitopes. Then the prediction was evaluated by T2 binding test. Results Five predicted highest score candidate epitopes were Hpa(525-533)

Hpa(353-361)

Hpa(277-285)

Hpa(400-408) and Hpa(405-413). The results of molecular simulations showed that the 5 candidate peptides could effectively bind with HLA-A2.1. Further peptide binding assay demonstrated that these 5 candidate peptides bound to HLA-A2.1 with high affinity. Hpa (525-533)

Hpa(277-285) and Hpa(405- 413) induced heparanase-specific immune responses against KATO-Ⅲ gastric tumor cells

which was both positive for heparanase and HLA-A2. Conclusion Hpa(525-533)

Hpa(277-285) and Hpa(405-413) might be the HLA-A2.1- restricted epitope of Hpa.

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