Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells

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Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells

更新时间：2026-01-09

- Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells
- Journal of Army Medical University Vol. 29, Issue 21, Pages: 2019-2022(2007)
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  CLC： R394.3;R73-362;R735.7
- Published：2007
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柏秀娟,张绪清.MHCⅡ类反式激活因子在上调HepG2细胞HLAⅡ类分子表达中的作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(21):2019-2022.

　BAI Xiu-juan,ZHANG Xu-qing.Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(21):2019-2022.
柏秀娟,张绪清.MHCⅡ类反式激活因子在上调HepG2细胞HLAⅡ类分子表达中的作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(21):2019-2022. DOI：

　BAI Xiu-juan,ZHANG Xu-qing.Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(21):2019-2022. DOI：

摘要

目的探讨主要组织相容性复合物（major histocompatibility complex，MHC）Ⅱ类反式激活因子（class Ⅱ transactivator

CⅡTA）在调控HepG2细胞HLAⅡ类分子表达中的作用。方法将含CⅡTA cDNA的真核表达载体EBS-NPL-CⅡTA和不含CⅡTA cDNA的空载体EBS-NPL分别转染至HepG2细胞。用RT-PCR技术检测未经转染的HepG2细胞、转染EBS-NPL-CⅡTA及空载体EBS-NPL的HepG2细胞CⅡTA mRNA的表达，并用间接细胞免疫荧光技术及流式细胞技术检测其3种HLAⅡ类分子(HLA-DR、DP、DQ)的表达。结果未经转染的HepG2细胞和转染空载体EBS-NPL的HepG2细胞均无CⅡTA mRNA和HLAⅡ类分子的表达。转染EBS-NPL-CⅡTA后的HepG2细胞出现CⅡTA mRNA表达，并表达3种HLAⅡ类分子。结论 CⅡTA是调控HepG2细胞是否表达HLAⅡ类分子的关键因子，HepG2细胞不表达HLA-Ⅱ类分子与其缺乏CⅡTA表达有关，为进一步研究CⅡTA在肝癌治疗中的作用奠定基础。

Abstract

Objective To investigate the role of MHC class Ⅱ transactivator (CⅡTA) in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells. Methods Eukaryotic expression vector EBS-NPL-CⅡTA containing CⅡTA cDNA or the empty vector EBS-NPL was transfected into HepG2 cells respectively. CⅡTA mRNA and HLA class Ⅱ antigen (HLA-DR

DP

DQ) were respectively detected by RT-PCR

indirect cell immunofluorescence technique and flow cytometry in original HepG2 cells

HepG2 cells transfected with EBS-NPL-CⅡTA or the empty vector EBS-NPL. Results The expression of CⅡTA mRNA and HLA class Ⅱ antigen (HLA-DR

DP

DQ) were not observed in original HepG2 cells and HepG2 cells transfected with empty vector

but in the HepG2 cells transfected with EBS-NPL-CⅡTA. Conclusion CⅡTA is a switching factor of mastering the expression of HLA class Ⅱ antigen in HepG2 cells. The lack of CⅡTA expression in HepG2 cells contributes to no expression of HLAⅡ antigen.

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