Objective    To identify a novel full-length human pancreatic cancer-associated gene screened by cDNA microarray.     Methods    One novel full-length gene which overexpressed in the pancreatic cancer was sequenced and analyzed for bioinformatics. The expression of the novel gene in 12 specimens of pancreatic cancer and normal tumor-surrounding pancreatic tissues and 4 pancreatic cancer cell lines were detected by RT-PCR and Northern blot analysis.     Results    Bioinformatics analysis showed that the chromatosome of the novel gene localized to 4p15

and had an open frame of 501 base pair encoding 166 amino acids of protein. Its theoretical molecular weight was 18 293.23 and PI was 8.57.  This protein had more than 90% homologous human hypothetical protein FLJ90013 using BLASTp analysis

and may be a new member of FLJ90013 protein family. The novel gene expressed in 12 specimens of pancreatic cancer tissues and 4 pancreatic cancer cell lines

but not in normal pancreatic tissues. This result was just the same as the result of Northern blot analysis.     Conclusion    The analysis of gene expression profile of tumor based on cDNA microarray can realize high-throughput screening of the genes associated with the pancreatic cancer

and it can help to rapidly explore the gene function. The novel gene is associated with oncogenesis and development of pancreatic cancer

and might be potential tumor marker or molecular target for diagnosis and treatment of pancreatic cancer.