Effect of Dexamethasone on tumor volume and apoptosis of transplanted HeLa cells in mice

曾建华; 钟玲; 徐波

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Effect of Dexamethasone on tumor volume and apoptosis of transplanted HeLa cells in mice

更新时间：2026-01-09

- Effect of Dexamethasone on tumor volume and apoptosis of transplanted HeLa cells in mice
- Journal of Army Medical University Vol. 29, Issue 20, Pages: 1985-1987(2007)
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  CLC： R73-352;R737.33;R977.1
- Published：2007
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曾建华,钟玲,徐波.地塞米松对宫颈癌移植瘤生长及凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1985-1987.

　ZENG Jian-hua,ZHONG Ling,XU Bo.Effect of Dexamethasone on tumor volume and apoptosis of transplanted HeLa cells in mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(20):1985-1987.
曾建华,钟玲,徐波.地塞米松对宫颈癌移植瘤生长及凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1985-1987. DOI：

　ZENG Jian-hua,ZHONG Ling,XU Bo.Effect of Dexamethasone on tumor volume and apoptosis of transplanted HeLa cells in mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(20):1985-1987. DOI：

摘要

目的探讨不同时间使用地塞米松对宫颈癌HeLa细胞移植瘤生长及凋亡的影响。方法 BALB/c裸鼠皮下接种HeLa细胞，于成瘤后测量肿瘤直径，不同时间(第6天和第16天)开始经腹腔内注射相同剂量地塞米松(15 mg·kg

-1

·d

-1

)，连用10 d，对肿瘤进行透射电镜观察及原位细胞凋亡检测（Terminal deoxynucleotidyl Transferase BiotindUTP Nick End Labeling，TUNEL）凋亡小体数目。结果不同时间使用地塞米松体内注射均抑制宫颈癌HeLa细胞裸鼠移植瘤生长，诱导肿瘤细胞凋亡；不同时间药物注射肿瘤的体积大小有差异（P0.01）；早期注射抑瘤率高（94.36%），电镜下凋亡小体明显，TUNEL检测凋亡小体数目较多；晚期注射抑瘤率低（53.57%），只有早期凋亡表现，TUNEL检测凋亡小体数目较少

组间比较有显著性差异（P0.01）。结论不同时间地塞米松体内注射均能抑制肿瘤生长，诱导凋亡发生，并存在时间依从性。

Abstract

Objective To investigate the effects of dexamethasone on the growth of cervical cancer induced by HeLa cells transplanted into BALB/c mice. Methods HeLa cells were inoculated subcutaneously into BALB/c mice. Dexamethasone (15 mg·kg

-1

·d

-1

) was injected intraperitoneally from the 6th or the 16th day after tumor inoculation for 10 days continuously. Diameter of tumors was measured every two or three days. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL. Results Dexamethasone repressed the growth of cervical cancer and induced

tumor cell apoptosis

but the tumor volumes were of statistical difference between the two groups of different injection start time (P0.01). The inhibition rate of tumor in mice receiving Dexamethasone early was 94.36%; Obvious apoptotic bodies were observed under EM and the number of apoptotic bodies was more. The inhibition rate of tumor in mice receiving Dexamethasone late was 53.57%; Early apoptosis was observed and the number of apoptotic bodies was less. Conclusion Dexamethasone can repress cervical tumor growth and induce tumor cell apoptosis in a time-dependent manner.

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