Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro

夏珊; 李世荣; 刘剑毅; 曹川; 姚恒; 冯智

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Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro

更新时间：2026-01-09

- Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro
- Journal of Army Medical University Vol. 29, Issue 20, Pages: 1982-1984(2007)
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  CLC： R619+6;R285;Q954.6-33+2
- Published：2007
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夏珊,李世荣,刘剑毅,曹川,姚恒,冯智.大黄素对人增生性瘢痕成纤维细胞的增殖抑制及细胞内游离Ca2+的作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1982-1984.

　XIA Shan,LI Shi-rong,LIU Jian-yi,CAO Chuan,YAO Heng,FENG Zhi.Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(20):1982-1984.
夏珊,李世荣,刘剑毅,曹川,姚恒,冯智.大黄素对人增生性瘢痕成纤维细胞的增殖抑制及细胞内游离Ca2+的作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1982-1984. DOI：

　XIA Shan,LI Shi-rong,LIU Jian-yi,CAO Chuan,YAO Heng,FENG Zhi.Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(20):1982-1984. DOI：

摘要

目的对大黄素的防治瘢痕的生物学作用进行初步探索，为瘢痕的防治开辟新的途径。方法采集我科住院患者的增生性瘢痕标本，进行人增生性瘢痕成纤维细胞培养，分别以不同浓度的大黄素作用于细胞，以MTT法测试药物处理后细胞增殖规律，并以激光共聚焦显微镜观察细胞内[Ca

]

荧光强度的变化。结果在不同浓度组大黄素的处理下，人增生性瘢痕成纤维细胞的增殖呈现出抑制的趋势(P0.05)；细胞内[Ca

]

的荧光强度也明显上升，其幅度和药物浓度有一定的关联。结论大黄素对人增生性瘢痕成纤维细胞有显著的抑制作用，其机制可能和细胞内[Ca

]

水平的改变有关。

Abstract

Objective To investigate the effect of emodin in preventing and treating hypertrophic scar. Methods The hypertrophic scar samples were collected from 6 patients admitted to our hospital. The fibroblasts of hypertrophic scar were cultured and treated with emodin (respectively 20

60 μg/ml) in culture medium. The proliferation of fibroblasts was detected by MTT colorimestric assay and the fluorescence intensity of intracellular free calcium was investgated by confocal laser microscopy. Results The proliferation of fibroblasts was inhibited significantly after emodin treatment in a dose-dependent manner

while the fluorescence intensity of intracellular free calcuim was significantly increased. Conlusion Emodin can inhibit the proliferation of hypertrophic scar fibroblasts

which mechanism may be related to increasing intracellular calcium

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