Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells

李琴山; 刘洋; 冯赞杰; 张亚玲; 钱民章

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Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells

更新时间：2026-01-09

- Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells
- Journal of Army Medical University Vol. 29, Issue 17, Pages: 1682-1684(2007)
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  CLC： R329.26;R322.12;R344.81
- Published：2007
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李琴山,刘洋,冯赞杰,张亚玲,钱民章.单核细胞趋化蛋白-1对人脐静脉内皮细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1682-1684.

　LI Qin-shan,LIU Yang,FENG Zan-jie,ZHANG Ya-ling,QIAN Min-zhang.Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1682-1684.
李琴山,刘洋,冯赞杰,张亚玲,钱民章.单核细胞趋化蛋白-1对人脐静脉内皮细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1682-1684. DOI：

　LI Qin-shan,LIU Yang,FENG Zan-jie,ZHANG Ya-ling,QIAN Min-zhang.Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1682-1684. DOI：

摘要

目的研究单核细胞趋化蛋白-1（monocyte chemotactic protein-1

MCP-1）对人脐静脉内皮细胞(human umbilical vein endothelial cells

hUVEC)凋亡的影响。方法培养并鉴定人脐静脉内皮细胞；用不同浓度MCP-1（0.1、1.0、10、100 μg/L）对其作用24、48 h；先用MTT比色法观察细胞存活率、流式细胞技术检测细胞DNA含量及细胞周期观察其对hUVEC凋亡的影响。结果单核细胞趋化蛋白-1能诱导人脐静脉内皮细胞的凋亡(P0.05)，其效应随浓度和时间的增加而增强：MTT明显减低；细胞DNA含量减少，出现明显亚G

峰（凋亡细胞峰）。结论单核细胞趋化蛋白-1能诱导人脐静脉内皮细胞凋亡。

Abstract

Objective To study the effects of monocyte chemotactic protein-1(MCP-1) on the growth of human umbilical vein endothelial cells (hUVEC). Methods hUVEC were cultured in vitro and identified. hUVEC that grew vigorously were stimulated for 24 h or 48 h respectively with MCP-1(0.1

1.0

and 100 μg/L). The survival rate of hUVEC was first detected by MTT assay. The cell cycle and DNA content were detected and analyzed by flow cytometry. Results hUVEC were isolated from human umbilical veins and cultured

and then identified by immunofluorescence and immunohistochemistry with factor Ⅷ and KDR. MCP-1 induced the apoptosis of hUVEC in a dose-dependentmanner and a time-dependentmanner (P0.05). Compared with control group

MTT and chromosomal DNA content decreased significantly. Conclusion MCP-1 can induce the apoptosis of hUVEC.

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