Effect of FGFR3 transfection on CbfaⅠ and Collagen Ⅰ expression in mouse mesenchymal stem cells

刘志君; 陈林

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Effect of FGFR3 transfection on CbfaⅠ and Collagen Ⅰ expression in mouse mesenchymal stem cells

更新时间：2026-01-09

- Effect of FGFR3 transfection on CbfaⅠ and Collagen Ⅰ expression in mouse mesenchymal stem cells
- Journal of Army Medical University Vol. 29, Issue 14, Pages: 1396-1398(2007)
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  CLC： R329.2;R394-33;R394.2
- Published：2007
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刘志君,陈林.FGFR3转染小鼠骨髓间充质干细胞对CbfaⅠ和Collagen Ⅰ表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(14):1396-1398.

　LIU Zhi-jun,CHEN Lin.Effect of FGFR3 transfection on CbfaⅠ and Collagen Ⅰ expression in mouse mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(14):1396-1398.
刘志君,陈林.FGFR3转染小鼠骨髓间充质干细胞对CbfaⅠ和Collagen Ⅰ表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(14):1396-1398. DOI：

　LIU Zhi-jun,CHEN Lin.Effect of FGFR3 transfection on CbfaⅠ and Collagen Ⅰ expression in mouse mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(14):1396-1398. DOI：

摘要

目的观察腺病毒载体Ad-FGFR3转染对小鼠骨髓间充质干细胞（mesenchymal stem cells， MSCs）相关成骨基因CbfaⅠ与CollagenⅠ表达的影响。方法采用Ad-FGFR3与重组空载体Ad-GFP病毒上清转染小鼠MSCs

RT-PCR和Western blot等方法检测FGFR3 、CbfaⅠ与CollagenⅠ mRNA及蛋白的表达水平。结果 RT-PCR及Western blot检测结果显示Ad-FGFR3转染组MSCs FGFR3、CbfaⅠ与CollagenⅠ的表达显著增强（P0.05）。结论 Ad-FGFR3转染显著增强MSCs相关成骨基因CbfaⅠ和Collagen Ⅰ的表达。

Abstract

Objective To explore the effect of fibroblast growth factor receptor 3 (FGFR3) on the expressions of CbfaⅠ and Collagen Ⅰ in mesenchymal stem cells (MSCs) after Ad-FGFR3 transfection. Methods After Ad-FGFR3 and Ad-GFP were respectively transfected into mouse mesenchymal stem cells

the expression of FGFR3

CbfaⅠ and Collagen Ⅰ mRNAs and proteins were detected by RT-PCR and Western blot. Results The expressions of FGFR3

CbfaⅠ and Collagen Ⅰ were significantly higher in Ad-FGFR3 transfected MSCs than in Ad-GFP group. Conclusion FGFR3 can up-regulate the expression of CbfaⅠ and Collagen Ⅰ.

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