Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV<sub>3</sub>

吴晓娟; 姚珍薇; 唐均英; 姚陈果; 孙才新

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Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV₃

更新时间：2026-01-09

- Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV₃
- Journal of Army Medical University Vol. 29, Issue 17, Pages: 1670-1672(2007)
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  CLC： R454.1;R73-36;R737.31
- Published：2007
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吴晓娟,姚珍薇,唐均英,姚陈果,孙才新.纳秒级陡脉冲对人卵巢癌细胞SKOV3生长抑制及诱导凋亡作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1670-1672.

　WU Xiao-juan,YAO Zhen-wei,TANG Jun-ying,YAO Chen-guo,SUN Cai-xin.Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1670-1672.
吴晓娟,姚珍薇,唐均英,姚陈果,孙才新.纳秒级陡脉冲对人卵巢癌细胞SKOV3生长抑制及诱导凋亡作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1670-1672. DOI：

　WU Xiao-juan,YAO Zhen-wei,TANG Jun-ying,YAO Chen-guo,SUN Cai-xin.Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV3[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1670-1672. DOI：

摘要

目的探讨纳秒级陡脉冲对人卵巢癌细胞的生长抑制和凋亡诱导作用。方法采用人卵巢癌细胞SKOV，应用MTT比色法，倒置显微镜，荧光显微镜及Annexin-FITC技术等方法进行检测和观察。结果中(8.5 kV/cm)、高(10 kV/cm)场强处理组对SKOV细胞活性具有明显抑制作用，呈剂量依赖性，在处理后2～4 h内呈时间依赖性；倒置显微镜和荧光显微镜下观察到典型的凋亡细胞形态；流式细胞仪示，中、高场强处理组早期凋亡率分别为（22.21±2.71）%、（57.30±6.51）%，与对照组（3.04±0.44）%比较均有显著性差异。结论一定范围内的纳秒级陡脉冲能明显抑制SKOV

细胞增殖，亦诱导SKOV细胞凋亡。

Abstract

Objective To investigate the effect of nanosecond steep pulse on growth and apoptosis of human ovarian carcinoma cells in vitro. Methods MTT assay

invert microscopy，fluorescence microscopy and annexinV-FITC were used to identify the inhibitory effect of nanosecond steep pulse on cell proliferation and the induction of apoptosis in human ovarian carcinoma cell Line SKOV

. Results Nanosecond steep pulse with 10 and 12.5 kV/cm significantly inhibited the viability of SKOV

cells in a dose-dependent manner

and in a time-dependent manner within 2 to 4 h of nanosecond steep pulse treatment. The typical changes of SKOV3 cell apoptosis were observed by i

nvert microscopy and fluorescence microscopy. Early apoptotic rate of SKOV

treated with 10

12.5 kV/cm nanosecond steep pulse was（22.21±2.71）% and （57.30±6.51）%

respectively

significantly higher than those of the control group（3.04±0.44）% (P0.05). Conclusion Nanosecond steep pulse in certain range can inhibit the growth of SKOV

cells as well as induce the apoptosis.

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Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV3

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摘要

Abstract

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Growth inhibition and apoptosis induction of nanosecond steep pulse on human ovarian carcinoma cell line SKOV₃