Objective    To evaluate the inhibitory effect of Cubilin antisense RNA mediated by recombinant adenovirus vector on albumin overload in renal tubules in rats.     Methods    The rat models of adriamycin (ADR) induced nephritis were established. Cub vector group and ACub vector group underwent infusion via renal artery with pAdEasy-Cub

pAdEasy-ACub both marked by GFP gene respectively; control group and model group were given the same dose of normal saline in the same way. The GFP expression was observed by laser scanning confocal microscopy; the changes of uria protein in 24 hours and other biochemical

indicators were measured simultaneously; the pathologic changes of renal tubule interstitium were observed under light microscope

and the Cubilin expression and the albumin deposition in renal tubules by immunohistochemistry.     Results    On the 4th day after transfection

the expression of GFP gene was detected in kidney in Cub vector group and ACub vector group

reached peak on the 11

th

day

lasted to the end of experiment. Compared with other groups

urine protein in 24 hours in ACub vector group increased obviously (P0.01)

the pathologic changes of renal tubule interstitium were mildest

and the positive rate of Cubilin expression was the lowest. The significant positive correlation was found between Cubilin expression and albumin deposition (r=0.896

P0.01).     Conclusion    When recombinant adenovirus vector was injected through renal artery

the introduced gene expressed preferentially in kidney. The transfected Cubilin antisense RNA has significant inhibitory effect on albumin overload in renal tubules and efficiently decreases the renal interstitial injury induced by proteinuria.