Radiolabelling  VEGF125-136 with <sup>99</sup>Tc<sup>m</sup> and their biodistribution in mice

黄定德; 李前伟; 刘广元; 谢赣丰; 程绍钧

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Radiolabelling VEGF125-136 with ⁹⁹Tc^m and their biodistribution in mice

更新时间：2026-01-09

- Radiolabelling VEGF125-136 with ⁹⁹Tc^m and their biodistribution in mice
- Journal of Army Medical University Vol. 29, Issue 17, Pages: 1660-1662(2007)
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  CLC： R341;R817
- Published：2007
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黄定德,李前伟,刘广元,谢赣丰,程绍钧.99Tcm标记多肽VEGF125-136及其生物学分布[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1660-1662.

　HUANG Ding-de,LI Qian-wei,LIU Guang-yuan,XIE Gan-feng,CHENG Shao-jun.Radiolabelling VEGF125-136 with 99Tcm and their biodistribution in mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1660-1662.
黄定德,李前伟,刘广元,谢赣丰,程绍钧.99Tcm标记多肽VEGF125-136及其生物学分布[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1660-1662. DOI：

　HUANG Ding-de,LI Qian-wei,LIU Guang-yuan,XIE Gan-feng,CHENG Shao-jun.Radiolabelling VEGF125-136 with 99Tcm and their biodistribution in mice[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1660-1662. DOI：

摘要

目的探讨一种理想的

标记小分子多肽VEGF125-136的方法，并研究标记物的生物学分布。方法在合成多肽的过程中直接完成多肽VEGF125-136与双功能螯合剂MAG

的偶联，然后采用正交设计探讨

的最佳标记条件，并研究标记物稳定性和小鼠体内分布。结果 ①简化了偶联步骤，提高了偶联产率；②最佳标记条件下标记率约78%，经HPLC纯化后放化纯度＞95%，且在室温条件下稳定性好；③

VEGF125-136-MAG

在正常小鼠的血液中清除较快，在肾、肝中摄取较高。结论以MAG

为螯合剂用

标记VEGF125-136有良好的标记率。

Abstract

Objective To explore a better method for radiolabelling VEGF125-136 with

and investigate the biodistribution of radiolabeled VEGF125-136 in mice. Methods Chelant MAG

was directly conjugated with VEGF125-136 during the synthesis of VEGF125-136. The best labelling conditions of

stability in vitro and biodistribution in normal mice of

-VEGF125-136-MAG

were investigated. Results The couple method between MAG

and VEGF125-136 was simplified and couple efficiency was raised. VEGF125-136 was successfully rad

iolabeled by

and the average labelling efficiency reached 78%. Being purified by HPLC

the radiochemical purity of

-VEGF125-136-MAG

was higher than 95%

and

-VEGF125-136-MAG

was stable in vitro at room temperature. The biodistribution study of

-VEGF125-136-MAG

in normal mice showed that there was a high uptake in kidney and liver

and can be cleared quickly from blood. Conclusion These strategies for coupling and labelling VEGF125-136 and MAG

are simple and efficient.

-VEGF125-136-MAG

is stable in vitro and appears to be suitable for further experiments and clinic applications in future.

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Radiolabelling VEGF125-136 with 99Tcm and their biodistribution in mice

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Radiolabelling VEGF125-136 with ⁹⁹Tc^m and their biodistribution in mice