Objective    To study the condensation mechanism of aminoglucomannan (AGM) with plasmid DNA and some influencing factors in this process.      Methods    The polysaccharide-DNA complex was analyzed with agarose gel electrophoresis by observing the changes of DNA bands under various influencing conditions including different pH

ionic strength (NaCl concentration)

temperature and plasmid DNA sorts.     Results    The new bands appeared in the slow migration positions after reaction between AGM and plasmid DNA

and the changes of DNA bands content were dependent on AGM concentrations. The condensation degree could be effected by pH

temperature

and ionic strength.     Conclusion    Our study suggests that the AGM can be condensed with plasmid DNA in proper conditions.