Effects of quercetin on proliferation of lung cancer cell line A549 by down-regulating hTERT gene expression

王箭; 张鹏辉; 涂植光

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Effects of quercetin on proliferation of lung cancer cell line A549 by down-regulating hTERT gene expression

更新时间：2026-01-09

- Effects of quercetin on proliferation of lung cancer cell line A549 by down-regulating hTERT gene expression
- Journal of Army Medical University Vol. 29, Issue 19, Pages: 1852-1854(2007)
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  CLC： R285.5;R730.2;R734.2
- Published：2007
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王箭,张鹏辉,涂植光.槲皮素对肺腺癌A549细胞生长的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(19):1852-1854.

　WANG Jian,ZHANG Peng-hui,TU Zhi-guang.Effects of quercetin on proliferation of lung cancer cell line A549 by down-regulating hTERT gene expression[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1852-1854.
王箭,张鹏辉,涂植光.槲皮素对肺腺癌A549细胞生长的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(19):1852-1854. DOI：

　WANG Jian,ZHANG Peng-hui,TU Zhi-guang.Effects of quercetin on proliferation of lung cancer cell line A549 by down-regulating hTERT gene expression[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1852-1854. DOI：

摘要

目的观察槲皮素对肺腺癌A549细胞生长及对hTERT基因表达的影响。方法以台盼蓝拒染法计数肺腺癌细胞的生长抑制率，透射电镜和DNA Ladder实验了解A549细胞凋亡的发生；PCR-TRAP法检测端粒酶活性，实时RT-PCR检测hTERT mRNA表达。结果台盼蓝拒染法计数显示槲皮素抑制肺腺癌A549细胞增殖的作用明显，且呈剂量和时间依赖性

槲皮素处理48 h后的IC

为22.5 μmol/L。DNA Ladder实验和透射电镜形态学检测显示出细胞凋亡的特征性变化，细胞呈现核固缩、染色质边集、凋亡小体形成等典型凋亡表现。经槲皮素处理后肺腺癌A549细胞hTERT mRNA表达降低，端粒酶活性受抑制。结论槲皮素能抑制肺腺癌细胞的生长

呈时间、剂量依赖性，能诱导A549细胞凋亡，其抑制增生与诱导凋亡的机制可能与下调hTERT 基因表达，抑制端粒酶活性，破坏端粒稳定性有关。

Abstract

Objective To investigate the effects of quercetin on the growth of lung cancer cell line A549 and the expression of hTERT gene. Methods The number of viable cells was ascertained by trypan blue dye exclusion test. Morphological changes of apoptotic cells were observed by electronic microscopy and DNA ladder assay. The telomerase activity was analyzed by PCR-TRAP assay and hTERT mRNA expression was detected by quantitative RT-PCR. Results Quercetin had a significant inhibition on the proliferation of A549 cells in a dose-dependent manner. The IC

was 22.5 μmol/L after exposure to quercetin for 48 h. The resu

lts from electron microscopy and DNA ladder showed that apoptosis occurred in the A549 cells of treatment groups. The results of quantitative RT-PCR and PCR-TRAP revealed that the expression of hTERT mRNA was significantly inhibited by quercetin and telomerase activity was decreased. Conclusion Quercetin inhibits the growth of lung cancer cell line A549 in a dose-dependent manner

and induce their apoptosis. The down-regulated expression of hTERT

suppression of telomerase activity and destruction of telomere stability may all contribute to the mechanism of apoptosis induction.

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