Extraction and activity of anti-lipopolysaccharide component from densefruit pittany root-bark

郭毅斌; 曹红卫; 范莉; 杨大成; 鲁永玲; 吕根法; 郑江; 肖光夏

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Extraction and activity of anti-lipopolysaccharide component from densefruit pittany root-bark

更新时间：2026-01-09

- Extraction and activity of anti-lipopolysaccharide component from densefruit pittany root-bark
- Journal of Army Medical University Vol. 29, Issue 17, Pages: 1654-1656(2007)
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  CLC： R284.2;R285.5
- Published：2007
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郭毅斌,曹红卫,范莉,杨大成,鲁永玲,吕根法,郑江,肖光夏.白鲜皮抗内毒素活性物质的分离提取与活性研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1654-1656.

　GUO Yi-bin,CAO Hong-wei,FAN Li,YANG Da-cheng,LU Yong-ling,LU Gen-fa,ZHENG Jiang,XIAO Guang-xia.Extraction and activity of anti-lipopolysaccharide component from densefruit pittany root-bark[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1654-1656.
郭毅斌,曹红卫,范莉,杨大成,鲁永玲,吕根法,郑江,肖光夏.白鲜皮抗内毒素活性物质的分离提取与活性研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1654-1656. DOI：

　GUO Yi-bin,CAO Hong-wei,FAN Li,YANG Da-cheng,LU Yong-ling,LU Gen-fa,ZHENG Jiang,XIAO Guang-xia.Extraction and activity of anti-lipopolysaccharide component from densefruit pittany root-bark[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(17):1654-1656. DOI：

摘要

目的筛选与类脂A（lipid A）具有较高结合活性的中药，从中提取具有拮抗内毒素（lipopolysaccharide

LPS）活性的物质。方法采用生物传感技术，以lipid A为靶点，检测42种中药的水提物与lipid A的结合活性；以白鲜皮为研究对象，通过大孔吸附树脂、溶剂萃取、聚酰胺柱层析和高效液相色谱（HPLC）法，分离提取与lipid A结合活性较高的组分；动态比浊法鲎试验检测HPLC产物DPR-1～3对LPS（0.1 ng/ml）的中和作用；ELISA法检测DPR-2对LPS（100 ng/ml）刺激的RAW264.7细胞释放肿瘤坏死因子-α（TNF-α）及白细胞介素-6（IL-6）的影响。结果 8种中药水提物与lipid A具有较高的结合活性(RU＞100 arc/s)；从白鲜皮中逐步分离出与lipid A结合活性较高的组分，经HPLC法获得3种物质DPR-1～3；DPR-2对LPS具有一定的中和作用，在8 μg/ml浓度以上可显著抑制LPS诱导的RAW264.7细胞释放TNF-α和IL-6 (P0.05)，并具有剂量效应关系。结论从42种中药中筛选出与lipid A具有较高结合活性的中药白鲜皮，并从中分离提取出抗LPS活性物质DPR-2，其抗LPS活性可能与DPR2对LPS的中和活性有关。

Abstract

Objective To screen the Chinese herbs with high affinity for lipid A and extract the anti-lipopolysaccharide (LPS) compound from densefruit pittany root-bark (DPR). Methods Forty-two kinds of aqueous extracts of traditional Chinese medicine were screened to determine their binding to lipid A by the biosensor technology. The chemical components with high affinity for lipid A were gotten by macroporous adsorptive resins

solvent extraction

polyamide laminar analysis and high performance liquid chromatogram (HPLC) from DPR. The neutralization of HPLC products DPR1-3 for LPS （0.1 ng/ml） was detected by kinetic turbidimetric limulus test. The release of TNF-α and IL-6 in RAW264.7 cells after exposure to LPS (100 ng/ml) were detected by ELISA. Results Eight kinds of aqueous extracts including DPR displayed high affinity for lipid A (RU100 arc/s). Three chemical components DPR1-3 with high affinity for lipid A were gotten. DPR-2 neutralized LPS and inhibited the release of TNF-α and IL-6 in LPS-induced RAW264.7 cells in a dose-dependent manner. Conclusion Eight herbs possess the components with high affinity for lipid A. DPR-2

extracted from DPR

shows anti-LPS activity probably by binding lipid A and neutralizing LPS.

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