Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model

余抒; 顾江; 曾浩; 杨琴; 刘艳青; 张卫军; 罗萍; 王庆旭; 毛旭虎

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Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model

更新时间：2026-01-09

- Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model
- Journal of Army Medical University Vol. 29, Issue 20, Pages: 1933-1935(2007)
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  CLC： R-33;R378.21;R446.5
- Published：2007
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余抒,顾江,曾浩,杨琴,刘艳青,张卫军,罗萍,王庆旭,毛旭虎.肠出血性大肠埃希菌O157∶H7黏附HeLa细胞模型的建立[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1933-1935.

　YU Shu,GU Jiang,ZENG Hao,YANG Qin,LIU Yan-qing,ZHANG Wei-jun,LUO Ping,WANG Qing-xu,MAO Xu-hu.Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(20):1933-1935.
余抒,顾江,曾浩,杨琴,刘艳青,张卫军,罗萍,王庆旭,毛旭虎.肠出血性大肠埃希菌O157∶H7黏附HeLa细胞模型的建立[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1933-1935. DOI：

　YU Shu,GU Jiang,ZENG Hao,YANG Qin,LIU Yan-qing,ZHANG Wei-jun,LUO Ping,WANG Qing-xu,MAO Xu-hu.Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(20):1933-1935. DOI：

摘要

目的建立肠出血性大肠埃希菌O157∶H7体外黏附HeLa细胞模型，为进一步研究其致病机制奠定基础。方法以细菌黏附HeLa细胞数量为指标，分别评价细菌数量、细菌与细胞孵育时间对细菌黏附数量的影响，得到最佳条件，最后肌动蛋白荧光染色（FAS）试验鉴定模型。结果加入细菌为1×10

CFU，细菌与细胞孵育4 h时，黏附细胞的数量最佳，FAS检测发现该条件下细菌能够引起细胞特异性黏附、擦拭(attaching effacing

A/E) 损伤，模型建立成功。结论成功建立O157∶H7体外黏附HeLa细胞模型，为进一步研究其致病机制提供了条件。

Abstract

Objective To establish Enterohemorrhagic Escherichia coli (EHEC) O157∶H7 attached HeLa cell model for further study on its pathogenic mechanism. Methods HeLa cells and Escherichia coli O157∶H7 were incubated together in different condition (amount of bacteria

incubation time). In regard to the amount of bacteria attached

we decided the best condition for O157∶H7 to infect HeLa cells and identified the characterized A/E (attaching and effacing) lesion by FAS (fluorescence actin staining). Results The best condition is that 1×10

CFU O157∶H7 and HeLa cells were incubated together for 4 h

under which the specific A/E lesion can be observed. Conclusion EHEC O157∶H7 attached HeLa cell model has been successfully established.

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