Preparation of polyclonal antibody of mouse IFIT1

郭晓姝; 李洪涛; 方海立; 王军平; 粟永萍

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Preparation of polyclonal antibody of mouse IFIT1

更新时间：2026-01-09

- Preparation of polyclonal antibody of mouse IFIT1
- Journal of Army Medical University Vol. 29, Issue 15, Pages: 1462-1464(2007)
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  CLC： R392-33;R392.11
- Published：2007
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郭晓姝,李洪涛,方海立,王军平,粟永萍.小鼠IFIT1多克隆抗体的制备[J].陆军军医大学学报(原第三军医大学学报),2007,29(15):1462-1464.

　GUO Xiao-shu,LI Hong-tao,FANG Hai-li,WANG Jun-ping,SU Yong-ping.Preparation of polyclonal antibody of mouse IFIT1[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(15):1462-1464.
郭晓姝,李洪涛,方海立,王军平,粟永萍.小鼠IFIT1多克隆抗体的制备[J].陆军军医大学学报(原第三军医大学学报),2007,29(15):1462-1464. DOI：

　GUO Xiao-shu,LI Hong-tao,FANG Hai-li,WANG Jun-ping,SU Yong-ping.Preparation of polyclonal antibody of mouse IFIT1[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(15):1462-1464. DOI：

摘要

目的获取小鼠IFIT1(Interferon-induced protein with tetratricopetide repeats 1 )特异性多克隆抗体。方法扩增培养高效表达IFIT1的重组子pMAL-C

X-IFIT1，超声破碎后的细胞上清过Amylose Pre-packed column亲和层析柱，将所得纯化的融合蛋白MBP-IFIT1作为抗原，添加福氏佐机剂后免疫兔，收集抗血清，用Western blot和ELISA方法测定抗血清特异性反应和效价。结果纯化的MBP-IFIT1可诱导兔产生特异性免疫应答，所得抗体能够特异性识别融合蛋白中的IFIT1，ELISA结果显示其效价为1∶12 800。结论 MBP-IFIT1具有良好的抗原性，以该融合蛋白为抗原免疫兔成功制备出IFIT1特异性多克隆抗体。

Abstract

Objective To acquire specific polyclonal antibody of mouse IFIT1 (Interferon-induced protein with tetratricopetide repeats 1). Methods The single clone of pMAL-C2X-IFIT1

confirmed previously to highly express MBP-IFIT1 fusion protein

was cultivated and amplified. After ultrasonic fragmentation and centrifugation

the MBP-IFIT1 fusion protein was purified from the supernatant by using the Amylose Pre-packed column. Then

the MBP-IFIT1 fusion protein

mixed with Freund’s adjuvant

was injected into rabbits twice with two-week interval. The rabbit serum was collected

then its anti-IFIT1 titer was detected by ELISA

and its anti-IFIT1 specific reaction was detected by Western blot. Results The rabbit serum immunized with purified MBP-IFIT1 protein showed specific anti-IFIT1 ability

and its antibody titer was about 1∶12 800. Conclusion The purified MBP-IFIT1 fusion protein possesses favorable antigenicity

and highly specific polyclonal antibody of mouse IFIT1 can be obtained by using purified MBP-IFIT1 as antigen to immunize rabbit.

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