Elementary characterization on epitopes of anti-shiga toxinⅡB subunit monoclonal antibody

陈洪章; 曾浩; 毛旭虎; 罗萍; 余抒; 邹全明

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Elementary characterization on epitopes of anti-shiga toxinⅡB subunit monoclonal antibody

更新时间：2026-01-09

- Elementary characterization on epitopes of anti-shiga toxinⅡB subunit monoclonal antibody
- Journal of Army Medical University Vol. 29, Issue 19, Pages: 1831-1833(2007)
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  CLC： R392.12;R394-33
- Published：2007
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陈洪章,曾浩,毛旭虎,罗萍,余抒,邹全明.抗志贺毒素ⅡB亚单位单克隆抗体识别表位的初步鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(19):1831-1833.

　CHEN Hong-zhang,ZENG Hao,MAO Xu-hu,LUO Ping,YU Shu,ZOU Quan-ming.Elementary characterization on epitopes of anti-shiga toxinⅡB subunit monoclonal antibody[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1831-1833.
陈洪章,曾浩,毛旭虎,罗萍,余抒,邹全明.抗志贺毒素ⅡB亚单位单克隆抗体识别表位的初步鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(19):1831-1833. DOI：

　CHEN Hong-zhang,ZENG Hao,MAO Xu-hu,LUO Ping,YU Shu,ZOU Quan-ming.Elementary characterization on epitopes of anti-shiga toxinⅡB subunit monoclonal antibody[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1831-1833. DOI：

摘要

目的初步确定抗志贺毒素ⅡB亚单位（Stx2B）单克隆抗体（McAb）1H

、1H

、3E

识别的抗原表位。方法运用DNAstar Protean软件，对Stx2B(1～72 aa)全抗原进行表位预测；采用截短法分段构建重组质粒Stx2B50(23～72 aa)，Stx2B54(1～54 aa)。用IPTG诱导表达融合蛋白。对表达产物进行SDS-PAGE蛋白电泳及Western blot 分析。结果 1H

、1H

能够识别Stx2B和Stx2B50；3E

能够识别Stx2B、Stx2B50和Stx2B54。结论 McAb 1H

、1H

识别的抗原表位位于55～72 aa，McAb 3E

识别的抗原表位位于23～54 aa。

Abstract

Objective To identify the epitope domain of monoclonal antibody 1H

and 3E

against shiga toxinⅡB subunit(Stx2B). Methods The epitopes of Stx2B (1-72 aa) were predicted by the DNAstar Protean software. The recombinant plasmids of Stx2B50 (23-72 aa)

Stx2B54 (1-54 aa) were constructed and then transformed to E.coli BL21 (DE3) for expression under IPTG induction. The proteins were identified by SDS-PAGE and Western blotting. Results The monoclonal antibody 1H

and 1H

recognized antigen of Stx2B and Stx2B 50

while 3E

recognized Stx2B

Stx2B50

and Stx2B5

4. Conclusion The epitope of monoclonal antibody 1H

and 1H

is located at 55-72 aa and the epitope of 3E

is located at 23-54 aa.

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