Changes and significance of C-ski and Smad3 in rat skin fibroblast of radiation induced injury

刘霞; 李平; 张恩; 刘苹; 周元国

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Changes and significance of C-ski and Smad3 in rat skin fibroblast of radiation induced injury

更新时间：2026-01-09

- Changes and significance of C-ski and Smad3 in rat skin fibroblast of radiation induced injury
- Journal of Army Medical University Vol. 29, Issue 15, Pages: 1455-1457(2007)
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  CLC： R322.99;R394.2;R818.023
- Published：2007
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刘霞,李平,张恩,刘苹,周元国.C-ski和Smad3在大鼠皮肤成纤维细胞辐射性损伤中的变化及意义[J].陆军军医大学学报(原第三军医大学学报),2007,29(15):1455-1457.

　LIU Xia,LI Ping,ZHANG En,LIU Ping,ZHOU Yuan-guo.Changes and significance of C-ski and Smad3 in rat skin fibroblast of radiation induced injury[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(15):1455-1457.
刘霞,李平,张恩,刘苹,周元国.C-ski和Smad3在大鼠皮肤成纤维细胞辐射性损伤中的变化及意义[J].陆军军医大学学报(原第三军医大学学报),2007,29(15):1455-1457. DOI：

　LIU Xia,LI Ping,ZHANG En,LIU Ping,ZHOU Yuan-guo.Changes and significance of C-ski and Smad3 in rat skin fibroblast of radiation induced injury[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(15):1455-1457. DOI：

摘要

目的研究C-ski和Smad3在大鼠皮肤成纤维细胞辐射性损伤中的变化和意义。方法软X线照射诱导大鼠皮肤成纤维细胞辐射性损伤，Western blot和RT-PCR观察C-ski、Smad3蛋白和mRNA水平的变化，EMSA检测Smad3转录活性的变化。结果辐射后2 h，C-ski、Smad3蛋白和mRNA水平、Smad3转录活性无显著变化，4 h开始，C-ski表达逐渐下降，Smad3表达和转录活性逐渐升高，在8 h分别达到峰谷和峰顶。结论 C-ski和Smad3参与大鼠皮肤成纤维细胞辐射性损伤的病理过程，辐射后C-ski下降，Smad3升高，并导致Smad3转录活性增强，细胞增殖受抑，凋亡增加。

Abstract

Objective To clarify the changes and significance of C-ski and Smad3 in rat skin fibroblast exposed to radiation. Methods The radiation induced injury of rat skin fibroblast was made by exposure to Soft X ray. Protein and mRNA expressions of C-ski and Smad3 were detected by Western blot and RT-PCR. The transcriptional activity of Smad3 was observed by electrophoretic mobility shift assay (EMSA). Results At 2 h after radiation

there were no obvious changes in protein and mRNA expression levels of C-ski

Smad3 or the transcriptional activity of Smad3. At 4 h

the expression of C-ski began to gradually decrease

reaching the minimum at 8 h

and Smad3 and Smad3 transcriptional activity began to increase

peaking at 8 h. Conclusion C-ski and Smad3 are involved in the pathological process of radiation induced fibroblast injury. Radiation can decrease C-ski and increase Smad3 expression

resulting in elevated Smad3 transcription activity

therefore cell proliferation is repressed and cell apoptosis is increased.

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